EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.19.1.1 | flavodoxin | cytochrome c reductase activity of FPR is stimulated three times by the addition of flavodoxin | Rhodobacter capsulatus |
EC Number | Cloned (Comment) | Organism |
---|---|---|
1.18.1.2 | gene fpr, DNA and amino acid sequence determination and analysis, overexpression in Escherichia coli | Rhodobacter capsulatus |
1.19.1.1 | expression in Escherichia coli | Rhodobacter capsulatus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.18.1.2 | 0.8 | - |
NADPH | diaphorase activity, pH 8.0 | Rhodobacter capsulatus | |
1.19.1.1 | 0.009 | - |
ferricytochrome c | presence of flavodoxin, pH 8.0, temperature not specified in the publication | Rhodobacter capsulatus | |
1.19.1.1 | 0.08 | - |
NADPH | substrate ferricyanide, pH 8.0, temperature not specified in the publication | Rhodobacter capsulatus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.18.1.2 | soluble | - |
Rhodobacter capsulatus | - |
- |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.18.1.2 | 30000 | - |
about, gel filtration | Rhodobacter capsulatus |
1.19.1.1 | 30000 | - |
gel filtration | Rhodobacter capsulatus |
1.19.1.1 | 30138 | - |
- |
Rhodobacter capsulatus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.18.1.2 | reduced ferredoxin + NADP+ | Rhodobacter capsulatus | - |
oxidized ferredoxin + NADPH | - |
? | |
1.18.1.2 | reduced ferredoxin + NADP+ | Rhodobacter capsulatus 37b4 | - |
oxidized ferredoxin + NADPH | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.18.1.2 | Rhodobacter capsulatus | Q9L6V3 | - |
- |
1.18.1.2 | Rhodobacter capsulatus 37b4 | Q9L6V3 | - |
- |
1.19.1.1 | Rhodobacter capsulatus | Q9L6V3 | enzyme catalyzes reactions of both EC 1.18.1.2 and EC 1.19.1.1 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.18.1.2 | recombinant enzyme from Escherichia coli to homogeneity, native enzyme about 400fold to homogeneity | Rhodobacter capsulatus |
1.19.1.1 | - |
Rhodobacter capsulatus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.18.1.2 | methyl viologen + NADPH | diaphorase activity | Rhodobacter capsulatus | reduced methyl viologen + NADP+ | - |
? | |
1.18.1.2 | methyl viologen + NADPH | diaphorase activity | Rhodobacter capsulatus 37b4 | reduced methyl viologen + NADP+ | - |
? | |
1.18.1.2 | reduced ferredoxin + NADP+ | - |
Rhodobacter capsulatus | oxidized ferredoxin + NADPH | - |
? | |
1.18.1.2 | reduced ferredoxin + NADP+ | - |
Rhodobacter capsulatus 37b4 | oxidized ferredoxin + NADPH | - |
? | |
1.18.1.2 | reduced flavodoxin + NADP+ | - |
Rhodobacter capsulatus | oxidized flavodoxin + NADPH + H+ | - |
r | |
1.18.1.2 | reduced flavodoxin + NADP+ | - |
Rhodobacter capsulatus 37b4 | oxidized flavodoxin + NADPH + H+ | - |
r | |
1.19.1.1 | 2 ferricyanide + NADPH + H+ | - |
Rhodobacter capsulatus | 2 ferrocyanide + NADP+ + H+ | - |
? | |
1.19.1.1 | 2 ferricyanide + NADPH + H+ | - |
Rhodobacter capsulatus 37b4 | 2 ferrocyanide + NADP+ + H+ | - |
? | |
1.19.1.1 | 2 ferricytochrome c + NADPH + H+ | - |
Rhodobacter capsulatus | 2 ferrocytochrome c + NADP+ + H+ | - |
? | |
1.19.1.1 | additional information | enzyme additionally shows diaphorase activity which is induced by treatment with methyl viologen | Rhodobacter capsulatus | ? | - |
? | |
1.19.1.1 | additional information | enzyme additionally shows diaphorase activity which is induced by treatment with methyl viologen | Rhodobacter capsulatus 37b4 | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.18.1.2 | monomer | 1 * 30000, about, recombinant and native enzyme, SDS-PAGE | Rhodobacter capsulatus |
1.19.1.1 | monomer | 1 * 30000, SDS-PAGE, 1 * 30138, calculated | Rhodobacter capsulatus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.18.1.2 | ferredoxin (flavodoxin)-(reduced) nicotinamide adenine dinucleotide phosphate reductase | - |
Rhodobacter capsulatus |
1.18.1.2 | ferredoxin (flavodoxin)-NADP(H) reductase | - |
Rhodobacter capsulatus |
1.18.1.2 | FPR | - |
Rhodobacter capsulatus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.18.1.2 | 0.25 | - |
reduced flavodoxin | pH 8.0 | Rhodobacter capsulatus | |
1.18.1.2 | 7.2 | - |
NADPH | diaphorase activity, pH 8.0 | Rhodobacter capsulatus | |
1.19.1.1 | 0.25 | - |
ferricytochrome c | presence of flavodoxin, pH 8.0, temperature not specified in the publication | Rhodobacter capsulatus | |
1.19.1.1 | 7.2 | - |
NADPH | substrate ferricyanide, pH 8.0, temperature not specified in the publication | Rhodobacter capsulatus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.18.1.2 | 8 | - |
assay at | Rhodobacter capsulatus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.18.1.2 | FAD | prosthetic group, tightly bound, can be released by heat denaturation | Rhodobacter capsulatus | |
1.18.1.2 | NADP+ | - |
Rhodobacter capsulatus | |
1.18.1.2 | NADPH | - |
Rhodobacter capsulatus | |
1.19.1.1 | FAD | - |
Rhodobacter capsulatus |