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Literature summary extracted from

  • Divari, S.; Valetti, F.; Caposio, P.; Pessione, E.; Cavaletto, M.; Griva, E.; Gribaudo, G.; Gilardi, G.; Giunta, C.
    The oxygenase component of phenol hydroxylase from Acinetobacter radioresistens S13 (2003), Eur. J. Biochem., 270, 2244-2253.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.13.7 oxygenase component PHO Acinetobacter radioresistens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.13.7 11600
-
 (alphabetagamma)2, 2 * 54000 + 2 * 37800 + 2 * 11600, oxygenase component PHO, SDS-PAGE Acinetobacter radioresistens
1.14.13.7 37800
-
 (alphabetagamma)2, 2 * 54000 + 2 * 37800 + 2 * 11600, oxygenase component PHO, SDS-PAGE Acinetobacter radioresistens
1.14.13.7 54000
-
 (alphabetagamma)2, 2 * 54000 + 2 * 37800 + 2 * 11600, oxygenase component PHO, SDS-PAGE Acinetobacter radioresistens
1.14.13.7 207000
-
 oxygenase component PHO, gel filtration Acinetobacter radioresistens

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.7 Acinetobacter radioresistens
-
 S13
-
1.14.13.7 Acinetobacter radioresistens S13
-
 S13
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.13.7 oxygenase component PHO Acinetobacter radioresistens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.7 3,4-dimethylphenol + NADH + H+ + O2 85% of the activity with phenol Acinetobacter radioresistens 1,2-dihydroxy-3,4-dimethylbenzene + NAD+ + H2O
-
 ?
1.14.13.7 3,4-dimethylphenol + NADH + H+ + O2 85% of the activity with phenol Acinetobacter radioresistens S13 1,2-dihydroxy-3,4-dimethylbenzene + NAD+ + H2O
-
 ?
1.14.13.7 3-chlorophenol + NADH + H+ + O2 15% of the activity with phenol Acinetobacter radioresistens 4-chlorocatechol + NAD+ + H2O
-
 ?
1.14.13.7 3-chlorophenol + NADH + H+ + O2 15% of the activity with phenol Acinetobacter radioresistens S13 4-chlorocatechol + NAD+ + H2O
-
 ?
1.14.13.7 4-chlorophenol + NADH + H+ + O2
-
 Acinetobacter radioresistens 1,2-dihydroxy-4-methylbenzene + NAD+ + H2O
-
 ?
1.14.13.7 4-chlorophenol + NADH + H+ + O2
-
 Acinetobacter radioresistens S13 1,2-dihydroxy-4-methylbenzene + NAD+ + H2O
-
 ?
1.14.13.7 4-chlorophenol + NADH + H+ + O2 27% of the activity with phenol Acinetobacter radioresistens 4-chlorocatechol + NAD+ + H2O
-
 ?
1.14.13.7 4-fluorophenol + NADH + H+ + O2
-
 Acinetobacter radioresistens 1,2-dihydroxy-4-fluorobenzene + NAD+ + H2O
-
 ?
1.14.13.7 4-methylphenol + NADH + H+ + O2
-
 Acinetobacter radioresistens 1,2-dihydroxy-4-methylbenzene + NAD+ + H2O
-
 ?
1.14.13.7 m-cresol + NADH + H+ + O2 60% of the activity with phenol Acinetobacter radioresistens 4-methylcatechol + NAD+ + H2O
-
 ?
1.14.13.7 additional information no PHO activity with p-hydroxybenzoic acid, m-hydroxybenzoic acid, 2,3-dinitrophenol, 3,4-dichlorophenol, 2,4,5-trichlorophenol, 2,2'-dihydroxybiphenyl and L-Tyr Acinetobacter radioresistens ?
-
 ?
1.14.13.7 additional information no PHO activity with p-hydroxybenzoic acid, m-hydroxybenzoic acid, 2,3-dinitrophenol, 3,4-dichlorophenol, 2,4,5-trichlorophenol, 2,2'-dihydroxybiphenyl and L-Tyr Acinetobacter radioresistens S13 ?
-
 ?
1.14.13.7 o-cresol + NADH + H+ + O2 60% of the activity with phenol Acinetobacter radioresistens 3-methylcatechol + NAD+ + H2O
-
 ?
1.14.13.7 p-cresol + NADH + H+ + O2 60% of the activity with phenol Acinetobacter radioresistens 4-methylcatechol + NAD+ + H2O
-
 ?
1.14.13.7 phenol + NADH + H+ + O2
-
 Acinetobacter radioresistens catechol + NAD+ + H2O
-
 ?
1.14.13.7 phenol + NADH + H+ + O2
-
 Acinetobacter radioresistens S13 catechol + NAD+ + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.13.7 hexamer (alphabetagamma)2, 2 * 54000 + 2 * 37800 + 2 * 11600, oxygenase component PHO, SDS-PAGE Acinetobacter radioresistens

Synonyms

EC Number Synonyms Comment Organism
1.14.13.7 phenol hydroxylase multicomponent enzyme made up of three moieties: a reductase (PHR), and an oxygenase (PHO) and a regulative component Acinetobacter radioresistens
1.14.13.7 PHO oxygenase component of the phenyl hydroxylase Acinetobacter radioresistens
1.14.13.7 PHR reductase component of phenyl hydroxylase Acinetobacter radioresistens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.13.7 24
-
 and a second smaller peak at 32°C Acinetobacter radioresistens

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.14.13.7 20 37 20°C: about 70% of maximal activity, 24°C: maximal activity, 30°C: about 40% of maximal activity, 32°C: about 65% of maximal activity, 37°C: about 30% of maximal activity, oxygenase component PHO Acinetobacter radioresistens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.14.13.7 1980
-
 NADH oxygenase component PHO Acinetobacter radioresistens
1.14.13.7 1980
-
 phenol oxygenase component PHO Acinetobacter radioresistens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.13.7 7.5
-
 oxygenase component PHO Acinetobacter radioresistens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.14.13.7 6.5 8.5 pH 6.5: about 45% of maximal activity, pH 8.5: about 30% of maximal activity, oxygenase component PHO Acinetobacter radioresistens

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.7 NADH
-
 Acinetobacter radioresistens

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.14.13.7 Acinetobacter radioresistens oxygenase component PHO
-
 6.7