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Literature summary extracted from

  • Walsh, H.A.; Botting, N.P.
    Purification and biochemical characterization of some of the properties of recombinant human kynureninase (2002), Eur. J. Biochem., 269, 2069-2074.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.7.1.3 expression in Spodoptera frugiperda cells Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.7.1.3 3,7-dihydroxy-deamino-DL-kynurenine mixed inhibition Homo sapiens
3.7.1.3 D-kynurenine mixed inhibition Homo sapiens
3.7.1.3 L-kynurenine competitive inhibition, at low substrate concentrations; non-competitive inhibition, at higher levels of substrate Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.7.1.3 3
-
 3-hydroxy-L-kynurenine pH 7.9, at 37ºC Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.7.1.3 52400
-
 recombinant enzyme, MALDI-TOF MS Homo sapiens
3.7.1.3 52500
-
 native enzyme, 2 * 52500, disc gel electrophoresis Homo sapiens
3.7.1.3 95000
-
 native enzyme, disc gel electrophoresis without SDS and mercaptoethanol Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.7.1.3 3-hydroxy-L-kynurenine + H2O Homo sapiens L-tryptophan metabolism, biosynthesis of nicotinamide nucleotides 3-hydroxyanthranilate + L-alanine
-
 ?
3.7.1.3 L-kynurenine + H2O Homo sapiens
-
 anthranilate + L-alanine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.7.1.3 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.7.1.3 recombinant enzyme, ammonium sulfate precipitation, DEAE-Sepharose CL-6B affinity column, hydroxyapatite column, strong anion-exchange column at pH 8.6, strong cation-exchange column at pH 6.0 strong anion-exchange column at pH 6.0, 60fold Homo sapiens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.7.1.3 0.164
-
 pH 7.9, 37ºC Homo sapiens

Storage Stability

EC Number Storage Stability Organism
3.7.1.3 -80ºC, pyridoxal 5'-phosphate, more than 12 months, no loss of activity Homo sapiens
3.7.1.3 -80ºC, without pyridoxal 5'-phosphate, more than 12 months, no loss of activity Homo sapiens
3.7.1.3 4ºC, pyridoxal 5'-phosphate, up to 2 weeks, stable Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.7.1.3 3-hydroxy-L-kynurenine + H2O
-
 Homo sapiens 3-hydroxyanthranilate + L-alanine
-
 ?
3.7.1.3 3-hydroxy-L-kynurenine + H2O L-tryptophan metabolism, biosynthesis of nicotinamide nucleotides Homo sapiens 3-hydroxyanthranilate + L-alanine
-
 ?
3.7.1.3 L-kynurenine + H2O
-
 Homo sapiens anthranilate + L-alanine
-
 ?

Subunits

EC Number Subunits Comment Organism
3.7.1.3 homodimer native enzyme, 2 * 52500, disc gel electrophoresis Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
3.7.1.3 L-kynurenine hydrolase
-
 Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.7.1.3 8.3
-
-
 Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
3.7.1.3 pyridoxal 5'-phosphate
-
 Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.7.1.3 0.0001
-
 3,7-dihydroxy-deamino-DL-kynurenine pH 7.9, at 37ºC Homo sapiens
3.7.1.3 0.012
-
 D-kynurenine pH 7.9, at 37ºC Homo sapiens
3.7.1.3 0.02
-
 L-kynurenine pH 7.9, at 37ºC, at low substrate concentrations Homo sapiens
3.7.1.3 0.055
-
 L-kynurenine pH 7.9, at 37ºC, at higher levels of substrate Homo sapiens