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Literature summary extracted from

  • Selmer, T.; Andrei, P.I.
    p-Hydroxyphenylacetate decarboxylase from Clostridium difficile. A novel glycyl radical enzyme catalysing the formation of p-cresol (2001), Eur. J. Biochem., 268, 1363-1372.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.1.83 (3,4-dihydroxyphenyl)acetate
-
 Clostridioides difficile
4.1.1.83 4-hydroxymandelate
-
 Clostridioides difficile
4.1.1.83 4-Hydroxyphenylacetamide competitive Clostridioides difficile
4.1.1.83 NaCl 800 mM, 50% inactivation Clostridioides difficile
4.1.1.83 O2 readily and irreversibly inhibited Clostridioides difficile

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.83 0.5
-
 (3,4-dihydroxyphenyl)acetate
-
 Clostridioides difficile
4.1.1.83 2.8
-
 (4-hydroxyphenyl)acetate
-
 Clostridioides difficile

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.83 105000
-
 alpha,alpha‘, the smaller unit is a C-terminally truncated form, 1 * 110000 + 1 * 105000, SDS-PAGE Clostridioides difficile
4.1.1.83 110000
-
 alpha,alpha‘, the smaller unit is a C-terminally truncated form, 1 * 110000 + 1 * 105000, SDS-PAGE Clostridioides difficile
4.1.1.83 200000
-
 gel filtration Clostridioides difficile

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.83 Clostridioides difficile
-
 DSMZ 1296T
-

Oxidation Stability

EC Number Oxidation Stability Organism
4.1.1.83 readily and irreversibly inhibited by O2 Clostridioides difficile

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.83
-
 Clostridioides difficile

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.1.83 0.315
-
-
 Clostridioides difficile

Storage Stability

EC Number Storage Stability Organism
4.1.1.83 0°C, 100 mM Tris/HCl, pH 7.5, 5 mM ammonium sulfate, 1 mM magnesium chloride, more than 90% of the activity is recovered after 5 days Clostridioides difficile

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.83 (3,4-dihydroxyphenyl)acetate + H+
-
 Clostridioides difficile 4-methylcatechol + CO2
-
 ?
4.1.1.83 (4-hydroxyphenyl)acetate + H+
-
 Clostridioides difficile 4-methylphenol + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.1.83 dimer alpha,alpha‘, the smaller unit is a C-terminally truncated form, 1 * 110000 + 1 * 105000, SDS-PAGE Clostridioides difficile

Synonyms

EC Number Synonyms Comment Organism
4.1.1.83 4-Hpd
-
 Clostridioides difficile
4.1.1.83 p-Hpd
-
 Clostridioides difficile
4.1.1.83 p-hydroxyphenylacetate decarboxylase
-
 Clostridioides difficile
4.1.1.83 pHPA decarboxylase
-
 Clostridioides difficile

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.1.1.83 30
-
 half-life: 15 min, in presence of 1 mM sodium sulfide and 5 mM ammonium sulfate Clostridioides difficile

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.1.1.83 6 9 0°C in presence of 1 mM sodium sulfide and 5 mM ammonium sulfate Clostridioides difficile

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.1.83 additional information indications for an as yet unidentified low molecular weight cofactor that is required for catalytic activity Clostridioides difficile

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.1.1.83 0.4
-
 (3,4-dihydroxyphenyl)acetate
-
 Clostridioides difficile
4.1.1.83 0.48
-
 4-hydroxymandelate
-
 Clostridioides difficile
4.1.1.83 0.7
-
 4-Hydroxyphenylacetamide
-
 Clostridioides difficile