Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Zamolodchikova T.S.; Vorotyntseva T.I.; Antonov V.K.
    Duodenase, a new serine protease of unusual specificity from bovine duodenal mucosa. Purification and properties (1995), Eur. J. Biochem., 227, 866-872.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.B3 alpha-N-tosyl-L-lysine chloromethane inhibitory effect on chymotrypsin-like and trypsin-like activity Bos taurus
3.4.21.B3 diisopropylphosphofluoridate irreversible inhibition Bos taurus
3.4.21.B3 inhibitor-chymotrypsin complex no inhibition of trypsin-like and chymotrypsin-like activity by EDTA, Ca2+, and benzamidine Bos taurus
3.4.21.B3 Kunitz type soybean trypsin inhibitor inhibitory effect on chymotrypsin-like and trypsin-like activity Bos taurus
3.4.21.B3 phenylmethanesulfonyl fluoride irreversible inhibition Bos taurus
3.4.21.B3 Trypsin inhibitor from bovine lungs, from Lima beans, inhibitory effect on chymotrypsin-like and trypsin-like activity Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.21.B3 0.1
-
 FMRF-NH2 pH 8.0, 37°C <2> Bos taurus
3.4.21.B3 0.28
-
 alpha-N-benzyloxycarbonyl-Ala-Ala-Ala-Arg-4-nitroanilide pH 8.0, 37°C Bos taurus
3.4.21.B3 0.8
-
 alpha-N-succinyl-Ala-Ala-Phe-4-nitroanilide pH 8.0, 37°C Bos taurus
3.4.21.B3 1
-
 LEEELKPLEEVLNL pH 8.0, 37°C Bos taurus
3.4.21.B3 1.1
-
 alpha-N-benzyloxycarbonyl-Ala-Ala-Ala-Arg-4-nitroanilide pH 8.0, 37°C Bos taurus
3.4.21.B3 3.2
-
 alpha-N-acetyl-Leu-Lys-Arg-4-nitroanilide pH 8.0, 37°C Bos taurus
3.4.21.B3 10.4
-
 alpha-N-acetyl-Leu-Leu-Arg-4-nitroanilide pH 8.0, 37°C Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.21.B3 29000
-
 gel filtration Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.21.B3 polypeptide + H2O Bos taurus enzyme belongs to the serine protease subfamily peptides
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.B3 Bos taurus P80219
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.B3 to homogeneity Bos taurus

Reaction

EC Number Reaction Comment Organism Reaction ID
3.4.21.B3 proteolytic cleavage of polypeptides to large and stable peptides, preferential cleavage sites: Lys-, Arg-, Tyr-, Phe-, Leu-, -Pro active site serine Bos taurus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.21.B3 duodenum
-
 Bos taurus
-
3.4.21.B3 mucosa
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.21.B3 53
-
 purified enzyme, substrate Ac-Leu-Lys-Arg-4-nitroanilide Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.B3 alpha-N-acetyl-Leu-Leu-Arg-4-nitroanilide + H2O
-
 Bos taurus alpha-N-acetyl-Leu-Leu-Arg + 4-nitroaniline
-
 ?
3.4.21.B3 alpha-N-acetyl-Leu-Lys-Arg-4-nitroanilide + H2O
-
 Bos taurus alpha-N-acetyl-Leu-Lys + Arg + 4-nitroaniline
-
 ?
3.4.21.B3 alpha-N-benzyloxycarbonyl-Ala-Ala-Ala-Arg-4-nitroanilide + H2O
-
 Bos taurus alpha-N-benzyloxycarbonyl-Ala-Ala-Ala-Arg + 4-nitroaniline
-
 ?
3.4.21.B3 alpha-N-benzyloxycarbonyl-Ala-Ala-Ile-Arg-Arg-4-nitroanilide + H2O
-
 Bos taurus alpha-N-benzyloxycarbonyl-Ala-Ala-Ile-Arg-Arg + 4-nitroaniline
-
 ?
3.4.21.B3 alpha-N-succinyl-Ala-Ala-Phe-4-nitroanilide + H2O
-
 Bos taurus alpha-N-succinyl-Ala-Ala-Phe + 4-nitroaniline
-
 ?
3.4.21.B3 bovine serum albumin + H2O enzyme cleaves the bonds Phe26-Ser27, Lys40-Leu41, Lys63-Ser64, Lys209-Ala210, Lys230-Leu231, Lys237-Val238, and Tyr409-Thr410 Bos taurus fragments of bovine serum albumin
-
 ?
3.4.21.B3 FMRF-NH2 + H2O
-
 Bos taurus ?
-
 ?
3.4.21.B3 glucagon + H2O enzyme cleaves the bonds Lys12-Arg13 and Arg18-Ala19 Bos taurus fragments of glucagon
-
 ?
3.4.21.B3 LEEELKPLEEVLNL + H2O
-
 Bos taurus LEEELKPL + EEVLNL
-
 ?
3.4.21.B3 polypeptide + H2O
-
 Bos taurus peptides
-
 ?
3.4.21.B3 polypeptide + H2O enzyme belongs to the serine protease subfamily Bos taurus peptides
-
 ?
3.4.21.B3 porcine proinsulin + H2O enzyme cleaves the bonds Lys-29-Thr30, Arg31-Arg32, Arg32-Glu33, Leu56-Ala57, Lys64-Arg65, and Arg66-Gly67 Bos taurus fragments of porcine proinsulin
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.21.B3 monomer 1 * 29000, SDS-PAGE Bos taurus

Synonyms

EC Number Synonyms Comment Organism
3.4.21.B3 duodenase I
-
 Bos taurus
3.4.21.B3 duodenase serine protease
-
 Bos taurus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.21.B3 37
-
-
 Bos taurus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.21.B3 0.6
-
 alpha-N-acetyl-Leu-Leu-Arg-4-nitroanilide pH 8.0, 37°C Bos taurus
3.4.21.B3 1.2
-
 alpha-N-acetyl-Leu-Lys-Arg-4-nitroanilide pH 8.0, 37°C Bos taurus
3.4.21.B3 3.6
-
 alpha-N-succinyl-Ala-Ala-Phe-4-nitroanilide pH 8.0, 37°C Bos taurus
3.4.21.B3 4.2
-
 FMRF-NH2 pH 8.0, 37°C Bos taurus
3.4.21.B3 14.4
-
 alpha-N-benzyloxycarbonyl-Ala-Ala-Ala-Arg-4-nitroanilide pH 8.0, 37°C Bos taurus
3.4.21.B3 60
-
 LEEELKPLEEVLNL pH 8.0, 37°C Bos taurus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.21.B3 7.9 8.2
-
 Bos taurus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.4.21.B3 0.002
-
 Kunitz soybean trypsin inhibitor pH 8.0, 37°C Bos taurus