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Literature summary extracted from

  • Bermek, H.; Yazici, H.; Ozturk, H.; Tamerler, C.; Jung, H.; Li, K.; Brown, K.M.; Ding, H.; Xu, F.
    Purification and characterization of manganese peroxidase from wood-degrading fungus Trichophyton rubrum LSK-27 (2004), Enzyme Microb. Technol., 35, 87-92.
No PubMed abstract available

General Stability

EC Number General Stability Organism
1.11.1.13 enzyme is very stable when the molar concentration of H2O2 is 10times of the concentration of the enzyme. When the H2O2 concentration is 250fold and 1000fold of the MnP concentration, the MnP activity decreases by 14% and 45%, respectively, in the first 15 min and remains the same thereafter. When the H2O2 concentration is 5000times of the enzyme concentration, the enzyme activity rapidly decreases in 30 min and then continues to decrease, but at a slower rate Trichophyton rubrum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.11.1.13 0.0064
-
 H2O2 reaction with Mn2+ Trichophyton rubrum
1.11.1.13 0.063
-
 Mn2+ reaction with H2O2 Trichophyton rubrum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.11.1.13 Mn2+ 2,2'-azino-bis(3-ethyl-benzothiazoline)-6-sulfonic acid, guaiacol and 2,6-dimethoxyphenol are oxidized at a faster rate in presence of Mn(II) than in absence of Mn(II) Trichophyton rubrum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.11.1.13 42000
-
 x * 42000, SDS-PAGE Trichophyton rubrum

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.13 Trichophyton rubrum
-
 LSK-27
-
1.11.1.13 Trichophyton rubrum LSK-27
-
 LSK-27
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.1.13
-
 Trichophyton rubrum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.13 H2O2 + 2,2'-azino-bis(3-ethyl)-benzothiazoline-6-sulfonic acid oxidized at a faster rate in presence of Mn(II) than in absence of Mn(II) Trichophyton rubrum H2O + ?
-
 ?
1.11.1.13 H2O2 + 2,2'-azino-bis(3-ethyl)-benzothiazoline-6-sulfonic acid oxidized at a faster rate in presence of Mn(II) than in absence of Mn(II) Trichophyton rubrum LSK-27 H2O + ?
-
 ?
1.11.1.13 H2O2 + 2,6-dimethoxyphenol oxidized at a faster rate in presence of Mn(II) than in absence of Mn(II) Trichophyton rubrum H2O + ?
-
 ?
1.11.1.13 H2O2 + 2,6-dimethoxyphenol oxidized at a faster rate in presence of Mn(II) than in absence of Mn(II) Trichophyton rubrum LSK-27 H2O + ?
-
 ?
1.11.1.13 H2O2 + guaiacol oxidized at a faster rate in presence of Mn(II) than in absence of Mn(II) Trichophyton rubrum H2O + ?
-
 ?
1.11.1.13 H2O2 + guaiacol oxidized at a faster rate in presence of Mn(II) than in absence of Mn(II) Trichophyton rubrum LSK-27 H2O + ?
-
 ?
1.11.1.13 Mn2+ + H2O2
-
 Trichophyton rubrum Mn3+ + H2O
-
 ?
1.11.1.13 Mn2+ + H2O2
-
 Trichophyton rubrum LSK-27 Mn3+ + H2O
-
 ?
1.11.1.13 additional information no oxidation of veratryl alcohol Trichophyton rubrum ?
-
 ?
1.11.1.13 additional information no oxidation of veratryl alcohol Trichophyton rubrum LSK-27 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.11.1.13 ? x * 42000, SDS-PAGE Trichophyton rubrum

Synonyms

EC Number Synonyms Comment Organism
1.11.1.13 MnP
-
 Trichophyton rubrum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.11.1.13 40
-
 60 min, stable Trichophyton rubrum
1.11.1.13 50
-
 20% decrease of activity after 20 min, no further loss of activity after 60 min Trichophyton rubrum
1.11.1.13 60
-
 80% loss of activity after 10 min, complete inactivation after 40 min Trichophyton rubrum
1.11.1.13 70
-
 complete inactivation after 5 min Trichophyton rubrum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.11.1.13 272.4
-
 H2O2 reaction with Mn2+ Trichophyton rubrum
1.11.1.13 304.8
-
 Mn2+ reactioin with H2O2 Trichophyton rubrum

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.11.1.13 Trichophyton rubrum isoelectric focusing
-
 8.2