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Literature summary extracted from

  • Cheesman, M.J.; Byron Kneller, M.; Rettie, A.E.
    Critical role of histidine residues in cyclohexanone monooxygenase expression, cofactor binding and catalysis (2003), Chem. Biol. Interact., 146, 157-164.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.13.22 expressed in Escherichia coli Acinetobacter sp.

Protein Variants

EC Number Protein Variants Comment Organism
1.14.13.22 H113Q only minor effects on activity Acinetobacter sp.
1.14.13.22 H162Q only minor effects on activity Acinetobacter sp.
1.14.13.22 H163Q 10fold lower turnover, doubling of Km Acinetobacter sp.
1.14.13.22 H200Q only minor effects on activity Acinetobacter sp.
1.14.13.22 H522Q only minor effects on activity Acinetobacter sp.
1.14.13.22 H59Q not expressed at significant levels Acinetobacter sp.
1.14.13.22 H96Q greatly reduced FAD content, reduced activity Acinetobacter sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.13.22 diethyl dicarbonate complete inhibition Acinetobacter sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.13.22 soluble
-
 Acinetobacter sp.
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.13.22 61600
-
 mass spectrometry Acinetobacter sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.13.22 cyclohexanone + NADPH + O2 Acinetobacter sp.
-
 hexano-6-lactone + NADP+ + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.22 Acinetobacter sp.
-
 NCIB 9871
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.13.22 recombinant protein Acinetobacter sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.13.22 1.4
-
 H163Q mutant enzyme Acinetobacter sp.
1.14.13.22 5.4
-
 H96Q mutant enzyme Acinetobacter sp.
1.14.13.22 15.9
-
 wild type enzyme Acinetobacter sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.22 cyclohexanone + NADPH + O2
-
 Acinetobacter sp. hexano-6-lactone + NADP+ + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.13.22 cyclohexanone:NADPH:oxygen oxidoreductase (lactone-forming)
-
 Acinetobacter sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.22 FAD
-
 Acinetobacter sp.
1.14.13.22 NADPH
-
 Acinetobacter sp.