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Literature summary extracted from

  • Yun, H.; Yang, Y.H.; Cho, B.K.; Hwang, B.Y.; Kim, B.G.
    Simultaneous synthesis of enantiomerically pure (R)-1-phenylethanol and (R)-alpha-methylbenzylamine from racemic alpha-methylbenzylamine using omega-transaminase/alcohol dehydrogenase/glucose dehydrogenase coupling reaction (2003), Biotechnol. Lett., 25, 809-814.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.6.1.18 synthesis enzymatic synthesis of enantiomerically pure (R)-1-phenylethanol and (R)-alpha-methylbenzylamine from racemic alpha-methylbenzylamine in a coupled assay using the omega-transaminase, alcohol dehydrogenase, and glucose dehydrogenase Vibrio fluvialis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.6.1.18 overexpression of the enzyme in Escherichia coli strain BL21 Vibrio fluvialis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.6.1.18 (R)-1-phenylethanol is a poor inhibitor which reduces enzyme activity by 10% at 25 mM Vibrio fluvialis
2.6.1.18 additional information no inhibition by D-glucose Vibrio fluvialis

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.18 Vibrio fluvialis
-
-
-
2.6.1.18 Vibrio fluvialis JS17
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.18 (S)-alpha-methylbenzylamine + pyruvate
-
 Vibrio fluvialis acetophenone + alanine
-
 ?
2.6.1.18 (S)-alpha-methylbenzylamine + pyruvate
-
 Vibrio fluvialis JS17 acetophenone + alanine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.6.1.18 omega-TA
-
 Vibrio fluvialis
2.6.1.18 omega-transaminase
-
 Vibrio fluvialis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.6.1.18 37
-
 assay at Vibrio fluvialis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.18 8
-
 assay at Vibrio fluvialis

Cofactor

EC Number Cofactor Comment Organism Structure
2.6.1.18 pyridoxal 5'-phosphate
-
 Vibrio fluvialis