Any feedback?
Literature summary extracted from
- Cloning and expression of sucrose phosphorylase gene from Bifidobacterium longum in E. coli and characterization of the recombinant enzyme (2003), Biotechnol. Lett., 25, 1211-1217.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 2.4.1.7 | cloning from genetic library, complementation of growth deficient Escherichia coli strain JM109, DNA and amino acid sequence determination and analysis, expression in Escherichia coli | Bifidobacterium longum |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 2.4.1.7 | 56000 | - |
x * 56000, recombinant enzyme, SDS-PAGE | Bifidobacterium longum |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 2.4.1.7 | Bifidobacterium longum | - |
sttrain SJ32, gene splP | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 2.4.1.7 | recombinant enzyme partially by anion exchange chromatography | Bifidobacterium longum |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 2.4.1.7 | 4-nitrophenyl alpha-D-glucopyranoside + H2O | hydrolytic activity | Bifidobacterium longum | 4-nitrophenol + alpha-D-glucose | - |
? |  |
| 2.4.1.7 | 4-nitrophenyl-alpha-D-galactopyranoside + H2O | hydrolytic activity | Bifidobacterium longum | 4-nitrophenol + alpha-D-galactose | - |
? | |
| 2.4.1.7 | additional information | the recombinant enzyme shows no activity with melibiose, melezitose, and raffinose, and exhibits transglucosylation activity in addition to hydrolytic activity | Bifidobacterium longum | ? | - |
? | |
| 2.4.1.7 | sucrose + phosphate | recombinant enzyme | Bifidobacterium longum | beta-D-fructose + alpha-D-glucose 1-phosphate | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 2.4.1.7 | ? | x * 56000, recombinant enzyme, SDS-PAGE | Bifidobacterium longum |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 2.4.1.7 | 30 | - |
assay at, activity with oligosaccharides | Bifidobacterium longum |
| 2.4.1.7 | 37 | - |
assay at, hydrolytic activity with 4-nitrophenyl-alpha-D-galactopyranoside and 4-nitrophenyl-alpha-D-glucopyranoside as substrates | Bifidobacterium longum |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 2.4.1.7 | 6.4 | - |
assay at | Bifidobacterium longum |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
