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Literature summary extracted from

  • Fischer, M.; Haase, I.; Feicht, R.; Schramek, N.; Kohler, P.; Schieberle, P.; Bacher, A.
    Evolution of vitamin B2 biosynthesis: riboflavin synthase of Arabidopsis thaliana and its inhibition by riboflavin (2005), Biol. Chem., 386, 417-428.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.9 phylogenetic analysis, highly effective expression in Escherichia coli by construction of a synthetic gene optimized for heterologous expression by exchange of 59 codons Arabidopsis thaliana

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.9 riboflavin sigmoidal product inhibition Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.9 additional information
-
 additional information kinetics, numerical simulation, recombinant enzyme Arabidopsis thaliana

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.9 21171
-
 3 * 21171, recombinant enzyme expressed from synthetic gene, electrospray mass spectrometry Arabidopsis thaliana
2.5.1.9 67500
-
 recombinant enzyme expressed from synthetic gene, sedimentation equilibrium centrifugation and gel filtration Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.9 6,7-dimethyl-8-(1-D-ribityl)lumazine Arabidopsis thaliana
-
 riboflavin + 5-amino-6-ribitylamino-2,4-(1H,3H)-pyrimidinedione
-
 ?
2.5.1.9 additional information Arabidopsis thaliana vitamin B2 biosynthetic pathway evolution, hypothetical gene duplication conductive to the 2-domain architecture occurring very early in evolution ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.9 Arabidopsis thaliana
-
 AY604007, GenBank synthetic gene
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.9 recombinant enzyme from Escherichia coli Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.9 6,7-dimethyl-8-(1-D-ribityl)lumazine
-
 Arabidopsis thaliana riboflavin + 5-amino-6-ribitylamino-2,4-(1H,3H)-pyrimidinedione
-
 ?
2.5.1.9 additional information vitamin B2 biosynthetic pathway evolution, hypothetical gene duplication conductive to the 2-domain architecture occurring very early in evolution Arabidopsis thaliana ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.9 trimer 3 * 21171, recombinant enzyme expressed from synthetic gene, electrospray mass spectrometry Arabidopsis thaliana

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.9 25
-
 assay at Arabidopsis thaliana

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.9 0.027
-
 6,7-dimethyl-8-(1-D-ribityl)lumazine 25°C, recombinant enzyme Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.9 7.3
-
 assay at Arabidopsis thaliana

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.5.1.9 additional information
-
 additional information inhibition kinetics, recombinant enzyme Arabidopsis thaliana