Literature summary extracted from
Smiley, J.A.; Kundracik, M.; Landfried, D.A.; Barnes, V.R., Sr.; Axhemi, A.A.
Genes of the thymidine salvage pathway: Thymine-7-hydroxylase from a Rhodotorula glutinis cDNA library and iso-orotate decarboxylase from Neurospora crassa (2005), Biochim. Biophys. Acta, 1723, 256-264.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
1.14.11.6 |
expressed in Escherichia coli BL21(DE3)Gold |
Rhodotorula glutinis |
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
1.14.11.6 |
37000 |
- |
calculated from deduced amino acid sequence |
Rhodotorula glutinis |
Natural Substrates/ Products (Substrates)
EC Number |
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
---|
1.14.11.6 |
thymine + 2-oxoglutarate + 2 O2 |
Rhodotorula glutinis |
conversion of thymine to uracil |
uracil-5-carboxylate + succinate + CO2 + H2O |
three step reaction with 5-hydroxymethyluracil and 5-formyluracil as intermediate products |
? |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
1.14.11.6 |
Rhodotorula glutinis |
Q66Q78 |
strain ATCC 2527 |
- |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
1.14.11.6 |
thymine + 2-oxoglutarate + 2 O2 |
- |
Rhodotorula glutinis |
uracil-5-carboxylate + succinate + CO2 + H2O |
three step reaction with 5-hydroxymethyluracil and 5-formyluracil as intermediate products |
? |
|
1.14.11.6 |
thymine + 2-oxoglutarate + 2 O2 |
conversion of thymine to uracil |
Rhodotorula glutinis |
uracil-5-carboxylate + succinate + CO2 + H2O |
three step reaction with 5-hydroxymethyluracil and 5-formyluracil as intermediate products |
? |
|