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Literature summary extracted from

  • Grishutin, S.G.; Gusakov, A.V.; Markov, A.V.; Ustinov, B.B.; Semenova, M.V.; Sinitsyn, A.P.
    Specific xyloglucanases as a new class of polysaccharide-degrading enzymes (2004), Biochim. Biophys. Acta, 1674, 268-281.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.151 additional information
-
 additional information KM-value for xyloglucan is 0.67 mg/ml Aspergillus japonicus
3.2.1.155 additional information
-
 additional information KM-value for xyloglucan is 0.3 mg/ml, KM-value for beta-glucan is 8 mg/ml, KM-value for carboxymethylcellulose is above 51 mg/mL Trichoderma reesei
3.2.1.155 additional information
-
 additional information KM-value for xyloglucan is 0.31 mg/ml, KM-value for beta-glucan is 18 mg/ml, KM-value for carboxymethylcellulose is above 50 mg/mL Chrysosporium lucknowense

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.151 32000
-
 x * 32000, SDS-PAGE Aspergillus japonicus
3.2.1.155 32000
-
 x * 32000, SDS-PAGE Aspergillus japonicus
3.2.1.155 78000
-
 x * 78000, SDS-PAGE Chrysosporium lucknowense

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.155 xyloglucan + H2O Aspergillus japonicus
-
 xyloglucan oligosaccharides
-
 ?
3.2.1.155 xyloglucan + H2O Chrysosporium lucknowense
-
 xyloglucan oligosaccharides
-
 ?
3.2.1.155 xyloglucan + H2O Trichoderma reesei
-
 xyloglucan oligosaccharides
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.151 Aspergillus japonicus
-
-
-
3.2.1.155 Aspergillus japonicus
-
-
-
3.2.1.155 Chrysosporium lucknowense
-
-
-
3.2.1.155 Trichoderma reesei Q7Z9M8
-
-
3.2.1.155 Trichoderma reesei Q7Z9M8 Cel74A; gene cel74, several enzyme forms
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.155 glycoprotein 2 putative N-glycosylation sites in Cel74A Trichoderma reesei
3.2.1.155 glycoprotein enzyme contains two putative N-glycosylation sites. There may be different forms of the enzyme resulting from O-glycosylation Trichoderma reesei
3.2.1.155 additional information Cel74A contains a signal peptide Trichoderma reesei

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.155 by ion exchange chromatography, to homogeneity Aspergillus japonicus
3.2.1.155 further purification of the commercial preparation, by ion exchange chromatography, to homogeneity Chrysosporium lucknowense
3.2.1.155 further purification of the commercial preparation, by ion exchange chromatography, to homogeneity Trichoderma reesei

Reaction

EC Number Reaction Comment Organism Reaction ID
3.2.1.155 Hydrolysis of (1->4)-D-glucosidic linkages in xyloglucans so as to successively remove oligosaccharides from the chain end. endo-type reaction mode, overview Aspergillus japonicus
a
3.2.1.155 Hydrolysis of (1->4)-D-glucosidic linkages in xyloglucans so as to successively remove oligosaccharides from the chain end. exo-type reaction mode, overview Chrysosporium lucknowense
a
3.2.1.155 Hydrolysis of (1->4)-D-glucosidic linkages in xyloglucans so as to successively remove oligosaccharides from the chain end. exo-type reaction mode, overview Trichoderma reesei
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.155 commercial preparation
-
 Chrysosporium lucknowense
-
3.2.1.155 commercial preparation
-
 Trichoderma reesei
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.151 additional information no activity with beta-glucan and carboxymethylcellulose Aspergillus japonicus ?
-
 ?
3.2.1.151 xyloglucan + H2O high specific activity towards tamarind xyloglucan Aspergillus japonicus xyloglucan oligosaccharides XXXG, XXLG and XLLG oligosaccharides ?
3.2.1.155 4-nitrophenyl xyloglucan derivatives + H2O artificial substrate Trichoderma reesei oligosaccharides
-
 ?
3.2.1.155 beta-glucan + H2O 8% of the activity with xyloglucan Chrysosporium lucknowense beta-glucan oligosaccharides
-
 ?
3.2.1.155 beta-glucan + H2O 8% of the activity with xyloglucan Trichoderma reesei beta-glucan oligosaccharides
-
 ?
3.2.1.155 carboxymethyl cellulose + H2O 5% of the activity with xyloglucan Chrysosporium lucknowense carboxymethyl cellulose oligosaccharides
-
 ?
3.2.1.155 carboxymethyl cellulose + H2O 6% of the activity with xyloglucan Trichoderma reesei carboxymethyl cellulose oligosaccharides
-
 ?
3.2.1.155 carboxymethylcellulose + H2O
-
 Trichoderma reesei carboxymethyl cellulose oligosaccharides
-
 ?
3.2.1.155 additional information substrate specificity, carboxymethylcellulose and barley beta-glucan are poor or no substrates Aspergillus japonicus ?
-
 ?
3.2.1.155 additional information substrate specificity, carboxymethylcellulose and barley beta-glucan are poor or no substrates Chrysosporium lucknowense ?
-
 ?
3.2.1.155 additional information substrate specificity, carboxymethylcellulose and barley beta-glucan are poor or no substrates Trichoderma reesei ?
-
 ?
3.2.1.155 tamarind xyloglucan + H2O highly specific for, exo-type reaction Trichoderma reesei oligosaccharides with end products XXXG, XXLG/XLXG, and XLLG ?
3.2.1.155 tamarind xyloglucan + H2O highly specific for, endo-type reaction Aspergillus japonicus oligosacchrides with end products XXXG, XXLG/XLXG, and XLLG ?
3.2.1.155 tamarind xyloglucan + H2O highly specific for, exo-type reaction Chrysosporium lucknowense oligosacchrides with end products XXXG, XXLG/XLXG, and XLLG ?
3.2.1.155 xyloglucan + H2O
-
 Aspergillus japonicus xyloglucan oligosaccharides
-
 ?
3.2.1.155 xyloglucan + H2O
-
 Chrysosporium lucknowense xyloglucan oligosaccharides
-
 ?
3.2.1.155 xyloglucan + H2O
-
 Trichoderma reesei xyloglucan oligosaccharides
-
 ?
3.2.1.155 xyloglucan + H2O
-
 Trichoderma reesei xyloglucan oligosaccharides XXXG, XXLG and XLLG oligosaccharides ?
3.2.1.155 xyloglucan + H2O the enzyme is a true endo-glucanase when it acts on linear substrates without bulky substituents in the polymer backbone. When the enzyme acts on polymeric substrate with bulky side chains, its mode of action is switched to exo-like Chrysosporium lucknowense xyloglucan oligosaccharides XXXG, XXLG and XLLG oligosaccharides ?

Subunits

EC Number Subunits Comment Organism
3.2.1.151 ? x * 32000, SDS-PAGE Aspergillus japonicus
3.2.1.155 ? x * 78000, SDS-PAGE Chrysosporium lucknowense
3.2.1.155 ? x * 32000, SDS-PAGE Aspergillus japonicus
3.2.1.155 ? x * 75000-105000, several enzyme forms, SDS-PAGE Trichoderma reesei
3.2.1.155 ? x * 75000-105000, isoelectrofocusing, there may be different forms of the enzyme resulting from O-glycosylation, SDS-PAGE Trichoderma reesei
3.2.1.155 More peptide fingerprinting Trichoderma reesei

Synonyms

EC Number Synonyms Comment Organism
3.2.1.155 Cel74A
-
 Trichoderma reesei
3.2.1.155 XG
-
 Aspergillus japonicus
3.2.1.155 XG
-
 Chrysosporium lucknowense
3.2.1.155 XG
-
 Trichoderma reesei
3.2.1.155 xyloglucanase
-
 Aspergillus japonicus
3.2.1.155 xyloglucanase
-
 Chrysosporium lucknowense
3.2.1.155 xyloglucanase
-
 Trichoderma reesei

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.155 40
-
 assay at Trichoderma reesei

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.151 40
-
 pH 5.0, stable for at least 24 h Aspergillus japonicus
3.2.1.151 50
-
 half-life: 24 h Aspergillus japonicus
3.2.1.151 60
-
 28 h, 50% loss of activity Aspergillus japonicus
3.2.1.155 40
-
 pH 5.0, stable for at least 24 h Aspergillus japonicus
3.2.1.155 40
-
 pH 5.0, stable for at least 24 h Chrysosporium lucknowense
3.2.1.155 40
-
 pH 5.0, stable for at least 24 h Trichoderma reesei
3.2.1.155 50
-
 half-life: 24 h Chrysosporium lucknowense
3.2.1.155 50
-
 half-life: 24 h Trichoderma reesei
3.2.1.155 50
-
 pH 5.0, half-life below 24 h Aspergillus japonicus
3.2.1.155 50
-
 pH 5.0, half-life below 24 h Chrysosporium lucknowense
3.2.1.155 50
-
 pH 5.0, half-life below 24 h Trichoderma reesei
3.2.1.155 60
-
 half-life: 15 min Trichoderma reesei
3.2.1.155 60
-
 half-life: 18 min Chrysosporium lucknowense
3.2.1.155 60
-
 pH 5.0, half-life 15 min Trichoderma reesei
3.2.1.155 60
-
 pH 5.0, half-life 18 min Chrysosporium lucknowense
3.2.1.155 60
-
 pH 5.0, half-life 28 h Aspergillus japonicus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.151 53
-
 xyloglucan
-
 Aspergillus japonicus
3.2.1.155 12
-
 beta-Glucan
-
 Trichoderma reesei
3.2.1.155 30
-
 beta-Glucan
-
 Chrysosporium lucknowense
3.2.1.155 46
-
 carboxymethylcellulose
-
 Trichoderma reesei
3.2.1.155 55
-
 xyloglucan
-
 Trichoderma reesei
3.2.1.155 99
-
 xyloglucan
-
 Chrysosporium lucknowense

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.151 5
-
-
 Aspergillus japonicus
3.2.1.155 5 6
-
 Aspergillus japonicus
3.2.1.155 5 6
-
 Chrysosporium lucknowense
3.2.1.155 5 6
-
 Trichoderma reesei
3.2.1.155 5.3
-
-
 Trichoderma reesei
3.2.1.155 6
-
-
 Chrysosporium lucknowense

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.151 3.8 6.1 more than 50% of maximal activity in the pH-range 3.8-6.1 Aspergillus japonicus
3.2.1.155 3.3 6.9 more than 50% of maximal activity in the pH-range 4.5-7.2 Trichoderma reesei
3.2.1.155 4.5 7.2 more than 50% of maximal activity in the pH-range 4.5-7.2 Chrysosporium lucknowense

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.151 5
-
 40°C, stable for at least 24 h Aspergillus japonicus
3.2.1.155 5
-
 40°C, stable for at least 24 h Chrysosporium lucknowense
3.2.1.155 5
-
 40°C, stable for at least 24 h Trichoderma reesei

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.151 Aspergillus japonicus isoelectrofocusing
-
 2.8
3.2.1.155 Aspergillus japonicus isoelectric focusing
-
 2.8
3.2.1.155 Chrysosporium lucknowense isoelectric focusing
-
 3.8
3.2.1.155 Chrysosporium lucknowense isoelectrofocusing
-
 3.8
3.2.1.155 Trichoderma reesei isoelectric focusing, several enzyme forms 4.3 4.1
3.2.1.155 Trichoderma reesei isoelectrofocusing, there may be different forms of the enzyme resulting from O-glycosylation 4.3 4.1