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Literature summary extracted from

  • Zako, T.; Ayabe, K.; Aburatani, T.; Kamiya, N.; Kitayama, A.; Ueda, H.; Nagamune, T.
    Luminescent and substrate binding activities of firefly luciferase N-terminal domain (2003), Biochim. Biophys. Acta, 1649, 183-189.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.13.12.7 CoA enhances luminescent activity of the wild-type enzyme about 2fold, highest activity at 1.2 mM CoA. For the N-terminal domain the effect of CoA is modest, 1.1fold enhancement, highest activity at 0.01 mM CoA. Addition of CoA at higher concentrations inhibits the luminescence activity Photinus pyralis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.12.7 Nn-terminal domain, expression in Escherichia coli Photinus pyralis

Protein Variants

EC Number Protein Variants Comment Organism
1.13.12.7 DELTA438-550 the purified N-terminal domain 1-437 has luminescence activity by itself, and binds to substrates ATP and luciferin with reduced affinity Photinus pyralis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.12.7 CoA above 0.1 mM, N-terminal domain Photinus pyralis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.12.7 0.0072
-
 Luciferin wild-type enzyme Photinus pyralis
1.13.12.7 0.026
-
 Luciferin N-terminal domain Photinus pyralis
1.13.12.7 0.23
-
 ATP wild-type enzyme Photinus pyralis
1.13.12.7 6.9
-
 ATP N-terminal domain Photinus pyralis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.12.7 Mg2+ optimal concentration for wild-type enzyme and N-terminal domain: 10 mM Photinus pyralis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.12.7 49000
-
 x * 49000, N-terminal domain, SDS-PAGE Photinus pyralis
1.13.12.7 61200
-
 x * 61200, wild-type enzyme, SDS-PAGE Photinus pyralis

Organism

EC Number Organism UniProt Comment Textmining
1.13.12.7 Photinus pyralis
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.12.7 luciferin + O2 + ATP
-
 Photinus pyralis oxidized luciferin + CO2 + H2O + AMP + diphosphate + hv
-
 ?

Subunits

EC Number Subunits Comment Organism
1.13.12.7 ? x * 49000, N-terminal domain, SDS-PAGE Photinus pyralis
1.13.12.7 ? x * 61200, wild-type enzyme, SDS-PAGE Photinus pyralis