EC Number | Cloned (Comment) | Organism |
---|---|---|
1.8.3.2 | overexpression of wild-type and mutant liver enzymes in Escherichia coli strain BL21(DE3) with a longer or shorter linker His-tag, overview | Homo sapiens |
1.8.3.3 | expressed in Escherichia coli | Homo sapiens |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.8.3.2 | C124A | site-directed mutagenesis, slightly reduced activity compared to the wild-type enzyme | Homo sapiens |
1.8.3.2 | C15A | site-directed mutagenesis, increased activity compared to the wild-type enzyme | Homo sapiens |
1.8.3.2 | C15A/C124A | site-directed mutagenesis, decreased activity compared to the wild-type enzyme | Homo sapiens |
1.8.3.2 | C15A/C74A/C85A/C124A | site-directed mutagenesis, increased activity compared to the wild-type enzyme | Homo sapiens |
1.8.3.2 | C74A/C85A | site-directed mutagenesis, decreased activity compared to the wild-type enzyme | Homo sapiens |
1.8.3.3 | C124A | reduced activity compared to wild type enzyme | Homo sapiens |
1.8.3.3 | C15A | reduced activity compared to wild type enzyme | Homo sapiens |
1.8.3.3 | C15A/C124A | reduced activity compared to wild type enzyme | Homo sapiens |
1.8.3.3 | C15A/C74A/C85A/C124A | reduced activity compared to wild type enzyme | Homo sapiens |
1.8.3.3 | C74A/C85A | reduced activity compared to wild type enzyme | Homo sapiens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.8.3.2 | additional information | - |
additional information | redox potential of wild-type and mutant enzymes | Homo sapiens | |
1.8.3.2 | 1.3 | - |
dithiothreitol | pH 7.5, 25°C, recombinant mutant C15A | Homo sapiens | |
1.8.3.2 | 1.7 | - |
dithiothreitol | pH 7.5, 25°C, recombinant mutant C15A/C74A/C85A/C124A | Homo sapiens | |
1.8.3.2 | 1.8 | - |
dithiothreitol | pH 7.5, 25°C, recombinant mutant C124A | Homo sapiens | |
1.8.3.2 | 2 | - |
dithiothreitol | pH 7.5, 25°C, recombinant mutants C74A/C85A and C15A/C124A | Homo sapiens | |
1.8.3.2 | 2.1 | - |
dithiothreitol | pH 7.5, 25°C, recombinant wild-type enzyme | Homo sapiens | |
1.8.3.3 | 0.01 | - |
cytochrome c | wild type enzyme | Homo sapiens | |
1.8.3.3 | 1.3 | - |
dithiothreitol | mutant enzyme C15A | Homo sapiens | |
1.8.3.3 | 1.7 | - |
dithiothreitol | mutant enzyme C15A/C74A/C85A/C124A | Homo sapiens | |
1.8.3.3 | 1.8 | - |
dithiothreitol | mutant enzyme C124A | Homo sapiens | |
1.8.3.3 | 2 | - |
dithiothreitol | mutant enzyme C15A/C124A | Homo sapiens | |
1.8.3.3 | 2 | - |
dithiothreitol | mutant enzyme C74A/C85A | Homo sapiens | |
1.8.3.3 | 2.1 | - |
dithiothreitol | wild type enzyme | Homo sapiens |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.8.3.2 | additional information | Homo sapiens | enzyme might communicate with the respiratory chain via the mediation of cytochrome c | ? | - |
? | |
1.8.3.2 | R-SH + O2 | Homo sapiens | - |
R-S-S-R + H2O2 | - |
ir |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.8.3.2 | Homo sapiens | - |
- |
- |
1.8.3.3 | Homo sapiens | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.8.3.2 | recombinant His-tagged wild-type and mutant liver enzymes from Escherichia coli strain BL21(DE3) | Homo sapiens |
1.8.3.3 | gel filtration | Homo sapiens |
EC Number | Renatured (Comment) | Organism |
---|---|---|
1.8.3.3 | in 8M urea | Homo sapiens |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.8.3.2 | liver | - |
Homo sapiens | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.8.3.2 | dithiothreitol + O2 | the enzyme forms large amounts of neutral semiquinone, which arises between flavin centers within the dimer, during aerobic turnover with DTT | Homo sapiens | dithiothreitol disulfide + H2O2 | - |
ir | |
1.8.3.2 | dithiothreitol + reduced cytochrome c | cytochrome c is about 100fold more effective than O2 as reducing cosubstrate | Homo sapiens | dithiothreitol disulfide + oxidized cytochrome c | - |
? | |
1.8.3.2 | additional information | enzyme might communicate with the respiratory chain via the mediation of cytochrome c | Homo sapiens | ? | - |
? | |
1.8.3.2 | R-SH + O2 | - |
Homo sapiens | R-S-S-R + H2O2 | - |
ir | |
1.8.3.2 | R-SH + O2 | disulfide bridge C15-C124 is not required for activity | Homo sapiens | R-S-S-R + H2O2 | - |
ir | |
1.8.3.3 | dithiothreitol + cytochrome c | - |
Homo sapiens | dithiothreitol disulfide + reduced cytochrome c | - |
? | |
1.8.3.3 | dithiothreitol + O2 | - |
Homo sapiens | dithiothreitol disulfide + H2O2 | - |
? | |
1.8.3.3 | R-SH + O2 | - |
Homo sapiens | R-S-S-R + H2O2 | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.8.3.2 | dimer | monomers are linked via a disulfide bridge C15-C124, which is not critical for dimer formation, structure modeling using the enzymes crystal structure | Homo sapiens |
1.8.3.3 | dimer | disulfide-bridged dimer (linked via C15-C124) with two free cysteine residues (C74 and 85) per monomer | Homo sapiens |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.8.3.2 | ALR | augmenter of liver regeneration | Homo sapiens |
1.8.3.2 | hepatopoietin | - |
Homo sapiens |
1.8.3.2 | More | enzyme belongs to the sulfhydryl oxidase/Quiescin Q6 family | Homo sapiens |
1.8.3.2 | QSOX | - |
Homo sapiens |
1.8.3.2 | quiescin-like flavin-dependent sulfhydryl oxidase | - |
Homo sapiens |
1.8.3.2 | sulfhydryl oxidase | - |
Homo sapiens |
1.8.3.3 | flavin-dependent sulfhydryl oxidase | - |
Homo sapiens |
1.8.3.3 | flavin-linked sulfhydryl oxidase | - |
Homo sapiens |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.8.3.2 | 25 | - |
assay at | Homo sapiens |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.8.3.2 | 0.83 | - |
dithiothreitol | pH 7.5, 25°C, recombinant mutant C74A/C85A | Homo sapiens | |
1.8.3.2 | 0.87 | - |
dithiothreitol | pH 7.5, 25°C, recombinant mutant C15A | Homo sapiens | |
1.8.3.2 | 0.9 | - |
dithiothreitol | pH 7.5, 25°C, recombinant mutant C124A | Homo sapiens | |
1.8.3.2 | 0.95 | - |
dithiothreitol | pH 7.5, 25°C, recombinant mutant C15A/C124A | Homo sapiens | |
1.8.3.2 | 1.1 | - |
dithiothreitol | pH 7.5, 25°C, recombinant wild-type enzyme | Homo sapiens | |
1.8.3.2 | 1.25 | - |
dithiothreitol | pH 7.5, 25°C, recombinant mutant C15A/C74A/C85A/C124A | Homo sapiens | |
1.8.3.3 | 0.83 | - |
dithiothreitol | mutant enzyme C74A/C85A | Homo sapiens | |
1.8.3.3 | 0.867 | - |
dithiothreitol | mutant enzyme C15A | Homo sapiens | |
1.8.3.3 | 0.9 | - |
dithiothreitol | mutant enzyme C124A | Homo sapiens | |
1.8.3.3 | 0.95 | - |
dithiothreitol | mutant enzyme C15A/C124A | Homo sapiens | |
1.8.3.3 | 1.1 | - |
dithiothreitol | wild type enzyme | Homo sapiens | |
1.8.3.3 | 1.25 | - |
dithiothreitol | mutant enzyme C15A/C74A/C85A/C124A | Homo sapiens | |
1.8.3.3 | 4.5 | - |
cytochrome c | wild type enzyme | Homo sapiens |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.8.3.2 | 7.5 | - |
assay at | Homo sapiens |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.8.3.2 | FAD | dependent on | Homo sapiens |