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Literature summary extracted from

  • Xu, X.; Kona, F.; Wang, J.; Lu, J.; Stemmler, T.; Gatti, D.L.
    The catalytic and conformational cycle of Aquifex aeolicus KDO8P synthase: role of the L7 loop (2005), Biochemistry, 44, 12434-12444.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.5.1.55 X-ray structure of wild-type enzyme shows that when both phosphoenolpyruvate and D-arabinose 5-phosphate bind, the active site becomes isolated from the environment due to a conformational change of the L7 loop. The structures of the R106G mutant, without substrates, and with phosphoenolpyruvate and phosphoenolpyruvate plus D-arabinose 5-phosphate bound reveal that in R106G closure of the L7 loop is impaired Aquifex aeolicus

Protein Variants

EC Number Protein Variants Comment Organism
2.5.1.55 R106G the closure of the L7 loop is impaired. The mutant enzyme shows a smaller KM-value for phosphoenolpyruvate, larger Ki-value and KM-value for D-arabinose 5-phosphate and smaller Ki-values for phosphate and 2-dehydro-3-deoxy-D-octonate 8-phosphate compared ti wild-type enzyme Aquifex aeolicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.55 2-dehydro-3-deoxy-D-octonate 8-phosphate mutant enzyme R106G is more severely inhibited than wild-type enzyme Aquifex aeolicus
2.5.1.55 D-arabinose 5-phosphate mutant enzyme R106G is less severely inhibited than wild-type enzyme Aquifex aeolicus
2.5.1.55 phosphate mutant enzyme R106G is more severely inhibited than wild-type enzyme Aquifex aeolicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.55 additional information
-
 additional information
-
 Aquifex aeolicus

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.55 Aquifex aeolicus O66496
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.55 phosphoenolpyruvate + D-arabinose 5-phosphate
-
 Aquifex aeolicus 2-dehydro-3-deoxy-D-octonate 8-phosphate + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.55 Kdo8P synthase
-
 Aquifex aeolicus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.55 0.32
-
 phosphoenolpyruvate mutant enzyme R106G Aquifex aeolicus
2.5.1.55 0.32
-
 D-arabinose 5-phosphate mutant enzyme R1096G Aquifex aeolicus
2.5.1.55 0.48
-
 phosphoenolpyruvate wild-type enzyme Aquifex aeolicus
2.5.1.55 0.48
-
 D-arabinose 5-phosphate wild-type enzyme Aquifex aeolicus