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Literature summary extracted from

  • Steinfels, E.; Orelle, C.; Fantino, J.R.; Dalmas, O.; Rigaud, J.L.; Denizot, F.; Di Pietro, A.; Jault, J.M.
    Characterization of YvcC (BmrA), a multidrug ABC transporter constitutively expressed in Bacillus subtilis (2004), Biochemistry, 43, 7491-7502.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
7.6.2.2 reserpine moderate stimulation of ATPase activity Bacillus subtilis
7.6.2.2 verapamil moderate stimulation of ATPase activity Bacillus subtilis
7.6.2.2 vinblastine moderate stimulation of ATPase activity, low concentrations of vinblastine stimulate the transport of Hoechst 33342, but higher concentrations prevent the transport of Hoechst 33342 Bacillus subtilis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
7.6.2.2 yvcC gene, overexpression in Escherichia coli C41(DE3) Bacillus subtilis

Protein Variants

EC Number Protein Variants Comment Organism
7.6.2.2 E504A mutant without ATPase activity Bacillus subtilis
7.6.2.2 E504Q mutant without ATPase activity Bacillus subtilis
7.6.2.2 K380A mutation in the Walker A motif, totally devoid of ATPase activity Bacillus subtilis
7.6.2.2 K380R mutation in the Walker A motif, deficient in ATPase activity and Hoechst 33342 transport Bacillus subtilis
7.6.2.2 additional information a yvcC knockout mutant shows a lower rate of ethidium bromide efflux than the wild-type strain Bacillus subtilis

Inhibitors

EC Number Inhibitors Comment Organism Structure
7.6.2.2 vanadate inhibits ATPase activity, 0.01 mM, 50% inhibition Bacillus subtilis
7.6.2.2 vinblastine higher concentrations of vinblastine prevent the transport of Hoechst 33342, but low concentrations stimulate the transport of Hoechst 33342 Bacillus subtilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
7.6.2.2 additional information
-
 additional information KD-values for several drugs Bacillus subtilis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
7.6.2.2 plasma membrane transmembrane protein Bacillus subtilis 5886
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
7.6.2.2 additional information Bacillus subtilis multidrug ABC transporter YvcC involved in multidrug resistance, the yvcC gene is constitutively expressed in Bacillus subtilis throughout its growth, the in vivo function is related to a protective role, the MDR pump might act as a housekeeping transporter to quickly cope with the presence of low levels of deleterious compounds, then, if the concentration of these compounds rose above a threshold limit, the expression of additional pumps could be turned on ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
7.6.2.2 Bacillus subtilis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
7.6.2.2
-
 Bacillus subtilis

Reaction

EC Number Reaction Comment Organism Reaction ID
7.6.2.2 ATP + H2O + xenobiotic[side 1] = ADP + phosphate + xenobiotic[side 2] positive cooperativity mechanism Bacillus subtilis
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7.6.2.2 7
-
 37°C, recombinant YvcC, ATPase activity Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.6.2.2 ATP + H2O + 2'-(4-ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5'-bis-1H-benzimidazole/in Hoechst 33342 Bacillus subtilis ADP + phosphate + 2'-(4-ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5'-bis-1H-benzimidazole/out
-
 ?
7.6.2.2 ATP + H2O + 7-aminoactinomycin D/in
-
 Bacillus subtilis ADP + phosphate + 7-aminoactinomycin D/out
-
 ?
7.6.2.2 ATP + H2O + bodipy-vinblastine/in
-
 Bacillus subtilis ADP + phosphate + bodipy-vinblastine/out
-
 ?
7.6.2.2 ATP + H2O + doxorubicin/in
-
 Bacillus subtilis ADP + phosphate + doxorubicin/out
-
 ?
7.6.2.2 ATP + H2O + ethidium bromide/in
-
 Bacillus subtilis ADP + phosphate + ethidium bromide/out
-
 ?
7.6.2.2 ATP + H2O + mitoxantrone/in
-
 Bacillus subtilis ADP + phosphate + mitoxantrone/out
-
 ?
7.6.2.2 ATP + H2O + xenobiotic/in multidrug ABC transporter YvcC, the high ATP hydrolysis proceeds via a positive cooperativity mechanism, two drug binding sites coexist on YvcC Bacillus subtilis ADP + phosphate + xenobiotic/out
-
 ?
7.6.2.2 additional information multidrug ABC transporter YvcC involved in multidrug resistance, the yvcC gene is constitutively expressed in Bacillus subtilis throughout its growth, the in vivo function is related to a protective role, the MDR pump might act as a housekeeping transporter to quickly cope with the presence of low levels of deleterious compounds, then, if the concentration of these compounds rose above a threshold limit, the expression of additional pumps could be turned on Bacillus subtilis ?
-
 ?
7.6.2.2 additional information no hydrolysis of 5’-adenylyl imidodiphosphate, study of YvcC interaction with different drugs Bacillus subtilis ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
7.6.2.2 BmrA YvcC is renamed BmrA, for Bacillus multidrug resistance ATP Bacillus subtilis
7.6.2.2 YvcC
-
 Bacillus subtilis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
7.6.2.2 25
-
 assay at, drug transport Bacillus subtilis
7.6.2.2 37
-
 assay at, ATPase activity Bacillus subtilis