Literature summary extracted from
Efimov, I.; McIntire, W.S.
A study of the spectral and redox properties and covalent flavinylation of the flavoprotein component of p-cresol methylhydroxylase reconstituted with FAD analogues (2004), Biochemistry, 43, 10532-10546.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
1.17.9.1 |
expression of the flavoprotein subunit with non-covalently attached FAD in Escherichia coli |
Pseudomonas putida |
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
1.17.9.1 |
Y384F |
strongly decreased activity |
Pseudomonas putida |
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
1.17.9.1 |
10000 |
- |
alpha, beta, 2 * 58000 + 2 * 10000, c-type cytochrome subunit PchC (beta) and two flavoprotein subunits PchF (alpha) |
Pseudomonas putida |
1.17.9.1 |
58000 |
- |
alpha, beta, 2 * 58000 + 2 * 10000, c-type cytochrome subunit PchC (beta) and two flavoprotein subunits PchF (alpha) |
Pseudomonas putida |
Natural Substrates/ Products (Substrates)
EC Number |
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
---|
1.17.9.1 |
4-cresol + acceptor + H2O |
Pseudomonas putida |
via intermediate 4-hydroxybenzylalcohol |
4-hydroxybenzaldehyde + reduced acceptor |
- |
? |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
1.17.9.1 |
Pseudomonas putida |
- |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
1.17.9.1 |
recombinant enzymes |
Pseudomonas putida |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
1.17.9.1 |
4-cresol + acceptor + H2O |
via intermediate 4-hydroxybenzylalcohol |
Pseudomonas putida |
4-hydroxybenzaldehyde + reduced acceptor |
- |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
1.17.9.1 |
tetramer |
alpha, beta, 2 * 58000 + 2 * 10000, c-type cytochrome subunit PchC (beta) and two flavoprotein subunits PchF (alpha) |
Pseudomonas putida |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
1.17.9.1 |
More |
consists of a c-type cytochrome subunit PchC and two flavoprotein subunits PchF |
Pseudomonas putida |
Turnover Number [1/s]
EC Number |
Turnover Number Minimum [1/s] |
Turnover Number Maximum [1/s] |
Substrate |
Comment |
Organism |
Structure |
---|
1.17.9.1 |
3.8 |
- |
4-Cresol |
Y384F mutant enzyme, pH 7, 25°C |
Pseudomonas putida |
|
1.17.9.1 |
121 |
- |
4-Cresol |
native enzyme, pH 7, 25°C |
Pseudomonas putida |
|
Cofactor
EC Number |
Cofactor |
Comment |
Organism |
Structure |
---|
1.17.9.1 |
FAD |
- |
Pseudomonas putida |
|