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Literature summary extracted from
- Characterization of a tungsten-substituted nitrogenase isolated from Rhodobacter capsulatus (2003), Biochemistry, 42, 3846-3857.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.18.6.1 | molybdate | induces MoFe protein expression | Rhodobacter capsulatus |  |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.18.6.1 | tungstate | 30% inhibition at 0.01 mM, 50% inhibition at 0.1 mM, 95% inhibition at 1 mM, inhibits MoFe protein expression | Rhodobacter capsulatus | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.18.6.1 | additional information | - |
additional information | EPR signals of Mo and of W containing cofactor, redox potentials | Rhodobacter capsulatus |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.18.6.1 | Iron | required, part of the MoFe protein cofactor | Rhodobacter capsulatus | |
| 1.18.6.1 | Molybdenum | required, part of the MoFe protein cofactor, can be partially, about 25%, substituted by tungsten, Mo content quantification, overview, Mo cannot be substituted by rhenium | Rhodobacter capsulatus | |
| 1.18.6.1 | additional information | rhenium cannot substitute for molybdenum in vitro, nor in vivo | Rhodobacter capsulatus | |
| 1.18.6.1 | Tungsten | can partially, about 25%, substitute for molybdenum, isolation of a tungsten-substituted enzyme after growth of the cells in tungsten-supplemented and molybdenum-deficient medium | Rhodobacter capsulatus | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.18.6.1 | 200000 | 250000 | tungsten-substituted enzyme component, gel filtration | Rhodobacter capsulatus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.18.6.1 | Rhodobacter capsulatus | - |
wild-type strain B10S, molybdenum-containing, nif-encoded enzyme variant | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.18.6.1 | wild-type and tungsten-substituted enzymes | Rhodobacter capsulatus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.18.6.1 | acetylene + ? | - |
Rhodobacter capsulatus | ethylene + ? | - |
? | |
| 1.18.6.1 | ethylene + ? | - |
Rhodobacter capsulatus | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.18.6.1 | tetramer | tungsten-substituted enzyme component | Rhodobacter capsulatus |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.18.6.1 | dinitrogenase | - |
Rhodobacter capsulatus |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.18.6.1 | iron-molybdenum cofactor | - |
Rhodobacter capsulatus | |
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