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Literature summary extracted from

  • Mast, N.; Norcross, R.; Andersson, U.; Shou, M.; Nakayama, K.; Bjorkhem, I.; Pikuleva, I.A.
    Broad substrate specificity of human cytochrome P450 46A1 which initiates cholesterol degradation in the brain (2003), Biochemistry, 42, 14284-14292.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.25 expression Escherichia coli, HEK293 cells transfected with CYP46A1 Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.25 cholesterol + [reduced NADPH-hemoprotein reductase] + H+ + O2 Homo sapiens initiates cholesterol degradation in the brain. In addition to the involvement in cholesterol homeostasis in the brain, this enzyme may participate in metabolism of neurosteroids and drugs that can cross the blood-brain barrier and are targeted to the central nervous system (24S)-24-hydroxycholesterol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.25 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.14.25 partial Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.14.25 brain
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.25 (24S)-24-hydroxycholesterol + [reduced NADPH-hemoprotein reductase] + O2
-
 Homo sapiens 24,25-dihydroxycholesterol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.25 (24S)-24-hydroxycholesterol + [reduced NADPH-hemoprotein reductase] + O2
-
 Homo sapiens 24,27-dihydroxycholesterol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.25 cholesterol + [reduced NADPH-hemoprotein reductase] + H+ + O2
-
 Homo sapiens (24S)-24-hydroxycholesterol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.25 cholesterol + [reduced NADPH-hemoprotein reductase] + H+ + O2 initiates cholesterol degradation in the brain. In addition to the involvement in cholesterol homeostasis in the brain, this enzyme may participate in metabolism of neurosteroids and drugs that can cross the blood-brain barrier and are targeted to the central nervous system Homo sapiens (24S)-24-hydroxycholesterol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.25 additional information the enzyme is able to carry out side chain hydroxylations of two endogenous C27-steroids with and without a double bond between C5-C6 (7R-hydroxycholesterol and cholestanol, respectively) and introduce a hydroxyl group on the steroid nucleus of the C21-steroid hormones with the C4-C5 double bond (progesterone and testosterone). P450 46A1 metabolizes xenobiotics carrying out dextromethorphan O-demethylation and N-demethylations, diclofenac 4'-hydroxylation, and phenacetin O-deethylation Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.14.25 cytochrome P450 46A1
-
 Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.14.25 NADPH
-
 Homo sapiens