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Literature summary extracted from

  • Terland, O.; Flatmark, T.
    Anion- and pH-dependent activation of the soluble form of dopamine beta-hydroxylase (2003), Biochem. J., 369, 675-679.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.17.1 acetate the enzyme is inactive inactive in absence of activating anion at pH 5.1-5.3, high catalytic activity in presence of 0.2 M acetate buffer Bos taurus
1.14.17.1 Cl- the enzyme is inactive inactive in absence of activating anion at pH 5.1-5.3, high catalytic activity in presence of 0.05-0.6 M Cl- in 50 mM Mes buffer. 0.6 M Cl- increases the optimum concentration of ferrocyanide from 0.25 mM to 2 mM Bos taurus
1.14.17.1 phosphate the enzyme is inactive inactive in absence of activating anion at pH 5.1-5.3, high catalytic activity in presence of 0.1 M phosphate buffer Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
1.14.17.1 Bos taurus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.17.1 adrenal medulla
-
 Bos taurus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.17.1 tyramine + ascorbate + ferrocyanide
-
 Bos taurus octopamine + dehydroascorbate + ?
-
 ?
1.14.17.1 tyramine + ascorbate + N,N,N',N'-tetramethyl-p-phenylenediamine
-
 Bos taurus octopamine + dehydroascorbate + ?
-
 ?
1.14.17.1 tyramine + ascorbate + N,N-dimethyl-p-phenylenediamine
-
 Bos taurus octopamine + dehydroascorbate + ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.17.1 DBH
-
 Bos taurus
1.14.17.1 dopamine beta-hydroxylase
-
 Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.17.1 6
-
 without anion activation or reaction with N,N,NĀ‘,NĀ‘-tetramethyl-p-phenylenediamine activated with 0.6 M Cl- Bos taurus