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Literature summary extracted from

  • Gravot, A.; Larbat, R.; Hehn, A.; Lievre, K.; Gontier, E.; Goergen, J.L.; Bourgaud, F.
    Cinnamic acid 4-hydroxylase mechanism-based inactivation by psoralen derivatives: cloning and characterization of a C4H from a psoralen producing plant-Ruta graveolens-exhibiting low sensitivity to psoralen inactivation (2004), Arch. Biochem. Biophys., 422, 71-80.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.91 expression in Saccharomyces cerevisiae Ruta graveolens
1.14.14.91 expression in Saccharomyces cerevisiae Petroselinum crispum
1.14.14.91 expression in Saccharomyces cerevisiae Helianthus tuberosus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.14.91 8-methoxypsoralen
-
 Helianthus tuberosus
1.14.14.91 8-methoxypsoralen
-
 Petroselinum crispum
1.14.14.91 8-methoxypsoralen
-
 Ruta graveolens
1.14.14.91 psoralen
-
 Helianthus tuberosus
1.14.14.91 psoralen
-
 Petroselinum crispum
1.14.14.91 psoralen irreversibly bound to the apoprotein. Inactivation is dependent on the presence of NADPH, time of pre-incubation, and inhibitor concentration Ruta graveolens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.14.91 1
-
 trans-cinnamate pH 7.4 Ruta graveolens
1.14.14.91 1
-
 trans-cinnamate pH 7.4 Helianthus tuberosus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.91 trans-cinnamate + [reduced NADPH-hemoprotein reductase] + O2 Petroselinum crispum key step in biosynthesis of phenylpropanoic compounds 4-hydroxycinnamate + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.91 Helianthus tuberosus Q04468
-
-
1.14.14.91 Petroselinum crispum Q43033
-
-
1.14.14.91 Ruta graveolens Q9AR74
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.14.91 leaf
-
 Ruta graveolens
-
1.14.14.91 leaf
-
 Petroselinum crispum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.91 trans-cinnamate + [reduced NADPH-hemoprotein reductase] + O2
-
 Ruta graveolens 4-hydroxycinnamate + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.91 trans-cinnamate + [reduced NADPH-hemoprotein reductase] + O2
-
 Petroselinum crispum 4-hydroxycinnamate + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.91 trans-cinnamate + [reduced NADPH-hemoprotein reductase] + O2
-
 Helianthus tuberosus 4-hydroxycinnamate + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.91 trans-cinnamate + [reduced NADPH-hemoprotein reductase] + O2 key step in biosynthesis of phenylpropanoic compounds Petroselinum crispum 4-hydroxycinnamate + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.14.91 cinnamic acid 4-hydroxylase
-
 Ruta graveolens
1.14.14.91 cinnamic acid 4-hydroxylase
-
 Petroselinum crispum
1.14.14.91 cinnamic acid 4-hydroxylase
-
 Helianthus tuberosus
1.14.14.91 CYP73A1
-
 Helianthus tuberosus
1.14.14.91 CYP73A10
-
 Petroselinum crispum
1.14.14.91 CYP73A32
-
 Ruta graveolens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.14.14.91 129.1
-
 trans-cinnamate pH 7.4 Helianthus tuberosus
1.14.14.91 161.8
-
 trans-cinnamate pH 7.4 Ruta graveolens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.14.14.91 0.0048
-
 8-methoxypsoralen pH 7.4 Helianthus tuberosus
1.14.14.91 0.0054
-
 psoralen pH 7.4 Helianthus tuberosus
1.14.14.91 0.0077
-
 psoralen pH 7.4 Ruta graveolens
1.14.14.91 0.00812
-
 psoralen pH 7.4 Petroselinum crispum
1.14.14.91 0.0142
-
 8-methoxypsoralen pH 7.4 Petroselinum crispum
1.14.14.91 0.0326
-
 8-methoxypsoralen pH 7.4 Ruta graveolens