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Literature summary extracted from
- Evidence that a type-2 NADH:quinone oxidoreductase mediates electron transfer to particulate methane monooxygenase in Methylococcus capsulatus (2002), Arch. Biochem. Biophys., 398, 32-40.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 7.1.1.2 | Amytal | 0.5 mM, 50% inhibition | Methylococcus capsulatus |  |
| 7.1.1.2 | additional information | no inhibition by rotenone up to 0.25 mM and flavone | Methylococcus capsulatus |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 7.1.1.2 | 0.1 | - |
menaquinone | - |
Methylococcus capsulatus | |
| 7.1.1.2 | 0.297 | - |
coenzyme Q10 | - |
Methylococcus capsulatus | |
| 7.1.1.2 | 0.489 | - |
duroquinone | - |
Methylococcus capsulatus | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 7.1.1.2 | 36000 | - |
1 * 36000, SDS-PAGE | Methylococcus capsulatus |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 7.1.1.2 | additional information | Methylococcus capsulatus | the enzyme mediates electron transfer to particulate methane monooxygenase | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 7.1.1.2 | Methylococcus capsulatus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 7.1.1.2 | - |
Methylococcus capsulatus |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 7.1.1.2 | 55 | - |
- |
Methylococcus capsulatus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 7.1.1.2 | additional information | the enzyme mediates electron transfer to particulate methane monooxygenase | Methylococcus capsulatus | ? | - |
? | |
| 7.1.1.2 | NADH + coenzyme Q10 | - |
Methylococcus capsulatus | ? | - |
? | |
| 7.1.1.2 | NADH + duroquinone | - |
Methylococcus capsulatus | NAD+ + ? | - |
? | |
| 7.1.1.2 | NADH + menadione | - |
Methylococcus capsulatus | NAD+ + menadiol | - |
? | |
| 7.1.1.2 | NADH + menaquinone + H+ | - |
Methylococcus capsulatus | NAD+ + menaquinol | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 7.1.1.2 | monomer | 1 * 36000, SDS-PAGE | Methylococcus capsulatus |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 7.1.1.2 | NDH-2 | - |
Methylococcus capsulatus |
| 7.1.1.2 | type-2 NADH:quinone oxidoreductase | - |
Methylococcus capsulatus |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 7.1.1.2 | FAD | - |
Methylococcus capsulatus | |
| 7.1.1.2 | additional information | no activity with NADPH | Methylococcus capsulatus | |
| 7.1.1.2 | NADH | no activity with NADPH | Methylococcus capsulatus | |
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Release 2023.1 (January 2023)
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