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Literature summary extracted from

  • Petersen, L.; Olewinski, R.; Salmon, P.; Connors, N.
    Novel proline hydroxylase activities in the pneumocandin-producing fungus Glarea lozoyensis responsible for the formation of trans 3- and trans 4-hydroxyproline (2003), Appl. Microbiol. Biotechnol., 62, 263-267.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.11.2 ascorbate required for optimal activity Glarea lozoyensis
1.14.11.2 dithiothreitol required for optimal activity Glarea lozoyensis
1.14.11.7 ascorbate required for full activity Glarea lozoyensis
1.14.11.7 dithiothreitol required for activity Glarea lozoyensis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.11.2 CoCl2 0.4 mM, complete inhibition Glarea lozoyensis
1.14.11.2 CuCl2 0.4 mM, 67% inhibition Glarea lozoyensis
1.14.11.2 EDTA 0.4 mM, 74% inhibition Glarea lozoyensis
1.14.11.2 MgSO4 0.4 mM, 30% inhibition Glarea lozoyensis
1.14.11.2 MnSO4 0.4 mM, 62% inhibition Glarea lozoyensis
1.14.11.2 NiCl2 0.4 mM, 98% inhibition Glarea lozoyensis
1.14.11.2 ZnSO4 0.4 mM, 84% inhibition Glarea lozoyensis
1.14.11.7 Co2+
-
 Glarea lozoyensis
1.14.11.7 Cu2+
-
 Glarea lozoyensis
1.14.11.7 Mg2+ slight inhibition Glarea lozoyensis
1.14.11.7 Mn2+ slight inhibition Glarea lozoyensis
1.14.11.7 Ni2+
-
 Glarea lozoyensis
1.14.11.7 Zn2+
-
 Glarea lozoyensis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.11.2 Ferrous sulfate required for activity Glarea lozoyensis
1.14.11.7 Iron required for activity Glarea lozoyensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.11.2 procollagen L-proline + 2-oxoglutarate + O2 Glarea lozoyensis
-
 procollagen trans-4-hydroxy-L-proline + succinate + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.11.2 Glarea lozoyensis
-
-
-
1.14.11.7 Glarea lozoyensis
-
 previously identified as Zalerion arboricola
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.11.7 mycelium
-
 Glarea lozoyensis
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.11.2 231
-
-
 Glarea lozoyensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.11.2 procollagen L-proline + 2-oxoglutarate + O2
-
 Glarea lozoyensis procollagen trans-4-hydroxy-L-proline + succinate + CO2
-
 ?
1.14.11.7 L-proline + 2-oxoglutarate + O2
-
 Glarea lozoyensis trans 3-hydroxy-L-proline + succinate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.11.2 P4H
-
 Glarea lozoyensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.11.2 25
-
-
 Glarea lozoyensis
1.14.11.7 25
-
-
 Glarea lozoyensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.11.2 7.5
-
-
 Glarea lozoyensis
1.14.11.7 7.5
-
-
 Glarea lozoyensis