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Literature summary extracted from

  • Uthoff, S.; Stoveken, T.; Weber, N.; Vosmann, K.; Klein, E.; Kalscheuer, R.; Steinbuchel, A.
    Thio wax ester biosynthesis utilizing the unspecific bifunctional wax ester synthase/acyl coenzyme A:diacylglycerol acyltransferase of Acinetobacter sp. strain ADP1 (2005), Appl. Environ. Microbiol., 71, 790-796.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.20 expression as soluble N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) Acinetobacter sp.
2.3.1.75 expression as soluble N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) Acinetobacter sp.

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.75 additional information the knockout mutant Acinetobacter sp. strain ADP1acr1omegaKm is unable to produce fatty alcohols Acinetobacter sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.75 soluble
-
 Acinetobacter baylyi ADP1
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.1.75 Mg2+
-
 Acinetobacter sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.20 additional information Acinetobacter sp. the bifunctional wax ester synthase/acyl coenzyme A (acyl-CoA):diacylglycerol acyltransferase from Acinetobacter sp. strain ADP1 mediates the biosyntheses of wax esters and triacylglycerols ?
-
 ?
2.3.1.20 additional information Acinetobacter sp. ADP1 the bifunctional wax ester synthase/acyl coenzyme A (acyl-CoA):diacylglycerol acyltransferase from Acinetobacter sp. strain ADP1 mediates the biosyntheses of wax esters and triacylglycerols ?
-
 ?
2.3.1.75 1-hexadecanethiol + palmitoyl-CoA Acinetobacter sp. mutant strain ADP1acr1omegaKm palmitic acid hexadecyl thio ester + CoA
-
 ?
2.3.1.75 1-hexadecanol + palmitoyl-CoA Acinetobacter sp. mutant strain ADP1acr1omegaKm palmitic acid hexadecyl ester + CoA
-
 ?
2.3.1.75 1-S-monopalmitoyloctanedithiol + palmitoyl-CoA Acinetobacter sp. mutant strain ADP1acr1omegaKm 1,8-S-dipalmitoyloctanedithiol + CoA
-
 ?
2.3.1.75 2 1,8-octanedithiol + 3 palmitoyl-CoA Acinetobacter sp. mutant strain ADP1acr1omegaKm 1-S-monopalmitoyloctanedithiol + 1,8-S-dipalmitoyloctanedithiol + 3 CoA 1-S-monopalmitoyloctanedithiol is the main product ?
2.3.1.75 additional information Acinetobacter sp. the bifunctional wax ester synthase/acyl coenzyme A (acyl-CoA):diacylglycerol acyltransferase from Acinetobacter sp. strain ADP1 mediates the biosyntheses of wax esters and triacylglycerols ?
-
 ?
2.3.1.75 additional information Acinetobacter sp. ADP1 the bifunctional wax ester synthase/acyl coenzyme A (acyl-CoA):diacylglycerol acyltransferase from Acinetobacter sp. strain ADP1 mediates the biosyntheses of wax esters and triacylglycerols ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.20 Acinetobacter sp.
-
 formerly Acinetobacter calcoaceticus ADP1
-
2.3.1.20 Acinetobacter sp. ADP1
-
 formerly Acinetobacter calcoaceticus ADP1
-
2.3.1.75 Acinetobacter baylyi ADP1 Q8GGG1 bifunctional wax ester synthase/acyl coenzyme A:diacylglycerol acyltransferase, EC 2.3.1.75 and EC 2.3.1.20 resp., expression in Escherichia coli with His-tag
-
2.3.1.75 Acinetobacter sp.
-
 formerly Acinetobacter calcoaceticus ADP1
-
2.3.1.75 Acinetobacter sp. ADP1
-
 formerly Acinetobacter calcoaceticus ADP1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.20 recombinant soluble N-terminally His-tagged enzyme 19.2fold from Escherichia coli strain BL21(DE3) by cation exchange and nickel affinity chromatography Acinetobacter sp.
2.3.1.75 recombinant soluble N-terminally His-tagged enzyme 19.2fold from Escherichia coli strain BL21(DE3) by cation exchange and nickel affinity chromatography Acinetobacter sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.75 0.165
-
 purified recombinant His-tagged enzyme Acinetobacter sp.
2.3.1.75 0.1652
-
 35°C, pH 7.4 Acinetobacter baylyi ADP1

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.20 additional information the bifunctional wax ester synthase/acyl coenzyme A (acyl-CoA):diacylglycerol acyltransferase from Acinetobacter sp. strain ADP1 mediates the biosyntheses of wax esters and triacylglycerols Acinetobacter sp. ?
-
 ?
2.3.1.20 additional information the bifunctional enzyme catalyzes the reactions of the diacylglycerol transferase, EC 2.3.1.20, and of the wax synthase, EC 2.3.1.75 Acinetobacter sp. ?
-
 ?
2.3.1.20 additional information the bifunctional wax ester synthase/acyl coenzyme A (acyl-CoA):diacylglycerol acyltransferase from Acinetobacter sp. strain ADP1 mediates the biosyntheses of wax esters and triacylglycerols Acinetobacter sp. ADP1 ?
-
 ?
2.3.1.20 additional information the bifunctional enzyme catalyzes the reactions of the diacylglycerol transferase, EC 2.3.1.20, and of the wax synthase, EC 2.3.1.75 Acinetobacter sp. ADP1 ?
-
 ?
2.3.1.75 1,8-octanedithiol + palmitoyl-CoA 59.3% the rate of hexadecanol, plus minor amounts of 1,8-dipalmitoyloctanedithiol Acinetobacter baylyi ADP1 1-S-monopalmitoyloctanedithiol + CoA
-
 ?
2.3.1.75 1-hexadecanethiol + palmitoyl-CoA 10.4% the rate of hexanol Acinetobacter baylyi ADP1 palmitic acid hexadecane thio ester + CoA
-
 ?
2.3.1.75 1-hexadecanethiol + palmitoyl-CoA
-
 Acinetobacter sp. palmitic acid hexadecyl thio ester + CoA
-
 ?
2.3.1.75 1-hexadecanethiol + palmitoyl-CoA mutant strain ADP1acr1omegaKm Acinetobacter sp. palmitic acid hexadecyl thio ester + CoA
-
 ?
2.3.1.75 1-hexadecanol + palmitoyl-CoA best substrate Acinetobacter sp. palmitic acid hexadecyl ester + CoA
-
 ?
2.3.1.75 1-hexadecanol + palmitoyl-CoA mutant strain ADP1acr1omegaKm Acinetobacter sp. palmitic acid hexadecyl ester + CoA
-
 ?
2.3.1.75 1-hexanol + palmitoyl-CoA
-
 Acinetobacter baylyi ADP1 hexyl palmitate + CoA
-
 ?
2.3.1.75 1-S-monopalmitoyloctanedithiol + palmitoyl-CoA 13.4% the rate of hexanol Acinetobacter baylyi ADP1 1,8-dipalmitoyloctanedithiol + CoA
-
 ?
2.3.1.75 1-S-monopalmitoyloctanedithiol + palmitoyl-CoA
-
 Acinetobacter sp. 1,8-S-dipalmitoyloctanedithiol + CoA
-
 ?
2.3.1.75 1-S-monopalmitoyloctanedithiol + palmitoyl-CoA mutant strain ADP1acr1omegaKm Acinetobacter sp. 1,8-S-dipalmitoyloctanedithiol + CoA
-
 ?
2.3.1.75 2 1,8-octanedithiol + 3 palmitoyl-CoA
-
 Acinetobacter sp. 1-S-monopalmitoyloctanedithiol + 1,8-S-dipalmitoyloctanedithiol + 3 CoA 1-S-monopalmitoyloctanedithiol is the main product ?
2.3.1.75 2 1,8-octanedithiol + 3 palmitoyl-CoA mutant strain ADP1acr1omegaKm Acinetobacter sp. 1-S-monopalmitoyloctanedithiol + 1,8-S-dipalmitoyloctanedithiol + 3 CoA 1-S-monopalmitoyloctanedithiol is the main product ?
2.3.1.75 2 1,8-octanedithiol + 3 palmitoyl-CoA
-
 Acinetobacter sp. ADP1 1-S-monopalmitoyloctanedithiol + 1,8-S-dipalmitoyloctanedithiol + 3 CoA 1-S-monopalmitoyloctanedithiol is the main product ?
2.3.1.75 additional information the bifunctional wax ester synthase/acyl coenzyme A (acyl-CoA):diacylglycerol acyltransferase from Acinetobacter sp. strain ADP1 mediates the biosyntheses of wax esters and triacylglycerols Acinetobacter sp. ?
-
 ?
2.3.1.75 additional information the bifunctional enzyme catalyzes the reactions of the diacylglycerol transferase, EC 2.3.1.20, and of the wax synthase, EC 2.3.1.75, substrate specificity of the wax ester synthase activity with alkanethiols as alternative acyl acceptors Acinetobacter sp. ?
-
 ?
2.3.1.75 additional information the bifunctional wax ester synthase/acyl coenzyme A (acyl-CoA):diacylglycerol acyltransferase from Acinetobacter sp. strain ADP1 mediates the biosyntheses of wax esters and triacylglycerols Acinetobacter sp. ADP1 ?
-
 ?
2.3.1.75 additional information the bifunctional enzyme catalyzes the reactions of the diacylglycerol transferase, EC 2.3.1.20, and of the wax synthase, EC 2.3.1.75, substrate specificity of the wax ester synthase activity with alkanethiols as alternative acyl acceptors Acinetobacter sp. ADP1 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.20 More cf. EC 2.3.1.75 Acinetobacter sp.
2.3.1.20 unspecific bifunctional wax ester synthase/acyl coenzyme A:diacylglycerol acyltransferase
-
 Acinetobacter sp.
2.3.1.20 WS/DGAT
-
 Acinetobacter sp.
2.3.1.75 More cf. EC 2.3.1.20 Acinetobacter sp.
2.3.1.75 unspecific bifunctional wax ester synthase/acyl coenzyme A:diacylglycerol acyltransferase
-
 Acinetobacter sp.
2.3.1.75 WS/DGAT
-
 Acinetobacter sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.75 35
-
 assay at Acinetobacter sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.75 7.4
-
 assay at Acinetobacter sp.