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Literature summary extracted from

  • Tosa, T.; Shibatani, T.
    Industrial applications of immobilized biocatalysts in Japan (1995), Ann. N. Y. Acad. Sci., 750, 364-375.
    View publication on PubMed

Application

EC Number Application Comment Organism
4.2.1.2 synthesis production of (S)-malic acid, which is used as an acidurant in fruit and vegetable juices, carbonated soft drinks, jams and candies, in amino acid infusions and for the treatment of hepatic malfunctioning [Brevibacterium] flavum
4.3.1.1 synthesis production of L-aspartic acid, which is used as an intermediate for aspartame, an artificial sweetener. It is also used as acidulant in pharmaceuticals and foods Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.2 [Brevibacterium] flavum
-
-
-
4.3.1.1 Escherichia coli
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.2 fumaric acid + H2O
-
[Brevibacterium] flavum (S)-malic acid
-
?
4.3.1.1 fumaric acid + NH3
-
Escherichia coli L-aspartic acid
-
?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.1.2 37
-
-
[Brevibacterium] flavum
4.3.1.1 37
-
-
Escherichia coli

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.2.1.2 6.5 8
-
[Brevibacterium] flavum