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Literature summary extracted from

  • Boisson, B.; Meinnel, T.
    A continuous assay of myristoyl-CoA:protein N-myristoyltransferase for proteomic analysis (2003), Anal. Biochem., 322, 116-123.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.3.1.97 analysis the automated assay can be used for proteomic studies to determine the myristoylation state of any protein Saccharomyces cerevisiae
2.3.1.97 analysis the automated assay can be used for proteomic studies to determine the myristoylation state of any protein Arabidopsis thaliana

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.97 palmitoyl-CoA
-
 Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.97 additional information
-
 additional information
-
 Saccharomyces cerevisiae
2.3.1.97 additional information
-
 additional information
-
 Arabidopsis thaliana
2.3.1.97 0.001
-
 myristoyl-CoA pH 8.0, 30°C, continuous assay Saccharomyces cerevisiae
2.3.1.97 0.0015
-
 myristoyl-CoA pH 8.0, 30°C, continuous assay Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.97 myristoyl-CoA + glycylpeptide Saccharomyces cerevisiae
-
 N-myristoylglycylpeptide + CoA
-
 ?
2.3.1.97 myristoyl-CoA + glycylpeptide Arabidopsis thaliana
-
 N-myristoylglycylpeptide + CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.97 Arabidopsis thaliana Q9LTR9
-
-
2.3.1.97 Saccharomyces cerevisiae
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.97 additional information
-
 continuous assay for proteomic analysis Saccharomyces cerevisiae
2.3.1.97 additional information
-
 continuous assay for proteomic analysis Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.97 myristoyl-CoA + glycylpeptide
-
 Saccharomyces cerevisiae N-myristoylglycylpeptide + CoA
-
 ?
2.3.1.97 myristoyl-CoA + glycylpeptide
-
 Arabidopsis thaliana N-myristoylglycylpeptide + CoA
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.97 myristoyl-CoA:protein N-myristoyltransferase
-
 Saccharomyces cerevisiae
2.3.1.97 myristoyl-CoA:protein N-myristoyltransferase
-
 Arabidopsis thaliana

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.97 additional information
-
 additional information
-
 Saccharomyces cerevisiae
2.3.1.97 additional information
-
 additional information
-
 Arabidopsis thaliana
2.3.1.97 0.234
-
 myristoyl-CoA pH 8.0, 30°C, continuous assay Arabidopsis thaliana
2.3.1.97 0.49
-
 myristoyl-CoA pH 8.0, 30°C, continuous assay Saccharomyces cerevisiae

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.3.1.97 additional information
-
 additional information
-
 Arabidopsis thaliana
2.3.1.97 0.0007
-
 palmitoyl-CoA substrate AtSOS3 + myristoyl-CoA Arabidopsis thaliana