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Literature summary extracted from

  • Uzunova, K.; Vassileva, A.; Kambourova, M.; Ivanova, V.; Spasova, D.; Mandeva, R.; Derekova, A.; Tonkova, A.
    Production and properties of a bacterial thermostable exo-inulinase (2001), Z. Naturforsch. C, 56, 1022-1028.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.80 nutrition microbial production of ultra high fructose glucose syrups Bacillus sp. (in: Bacteria)

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.80 extracellular
-
 Bacillus sp. (in: Bacteria)
-
-

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.80 Bacillus sp. (in: Bacteria)
-
-
-
3.2.1.80 Bacillus sp. (in: Bacteria) 11
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.80 no modification
-
 Bacillus sp. (in: Bacteria)

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.80 ultrafiltration, 1.3fold Bacillus sp. (in: Bacteria)

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.80 culture supernatant
-
 Bacillus sp. (in: Bacteria)
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.80 additional information
-
 19.0 ?mol/min/ml, for sucrose, pH 7, at 60ºC Bacillus sp. (in: Bacteria)
3.2.1.80 additional information
-
 2.0 ?mol/min/ml, for inulin, pH 7, at 60ºC Bacillus sp. (in: Bacteria)
3.2.1.80 additional information
-
 6.2 ?mol/min/ml, for raffinose, pH 7, at 60ºC Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.80 inulin + 5 H2O no activity with melizitose Bacillus sp. (in: Bacteria) 5 beta-D-fructofuranose + alpha-D-glucopyranose
-
 ?
3.2.1.80 inulin + 5 H2O no activity with melizitose Bacillus sp. (in: Bacteria) 11 5 beta-D-fructofuranose + alpha-D-glucopyranose
-
 ?
3.2.1.80 raffinose + H2O
-
 Bacillus sp. (in: Bacteria) beta-D-fructofuranose + O6-alpha-D-galactopyranosyl-alpha-D-glucopyranoside
-
 ?
3.2.1.80 raffinose + H2O
-
 Bacillus sp. (in: Bacteria) 11 beta-D-fructofuranose + O6-alpha-D-galactopyranosyl-alpha-D-glucopyranoside
-
 ?
3.2.1.80 sucrose + H2O
-
 Bacillus sp. (in: Bacteria) beta-D-fructofuranose + alpha-D-glucopyranose
-
 ?
3.2.1.80 sucrose + H2O
-
 Bacillus sp. (in: Bacteria) 11 beta-D-fructofuranose + alpha-D-glucopyranose
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.80 exoinulinase
-
 Bacillus sp. (in: Bacteria)

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.80 65
-
-
 Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.80 60
-
 without inulin, 73% of maximal activity after heating for 60 min Bacillus sp. (in: Bacteria)
3.2.1.80 60
-
 without inulin, 78% of maximal activity after heating for 15 min Bacillus sp. (in: Bacteria)
3.2.1.80 65
-
 with inulin, 92% of maximal activity after heating for 60 min Bacillus sp. (in: Bacteria)
3.2.1.80 65
-
 with inulin, 97% of maximal activity after heating for 15 min Bacillus sp. (in: Bacteria)
3.2.1.80 65
-
 without inulin, 17% of maximal activity after heating for 60 min Bacillus sp. (in: Bacteria)
3.2.1.80 65
-
 without inulin, 34% of maximal activity after heating for 15 min Bacillus sp. (in: Bacteria)
3.2.1.80 70
-
 with inulin, 56% of maximal activity after heating for 60 min Bacillus sp. (in: Bacteria)
3.2.1.80 70
-
 with inulin, 85% of maximal activity after heating for 15 min Bacillus sp. (in: Bacteria)
3.2.1.80 70
-
 without inulin, 2% of maximal activity after heating for 60 min Bacillus sp. (in: Bacteria)
3.2.1.80 70
-
 without inulin, 7% of maximal activity after heating for 15 min Bacillus sp. (in: Bacteria)

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.80 6.5 7 at pH 8.0, 58% of maximal activity Bacillus sp. (in: Bacteria)

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.80 5.5 7.5 95-100% of maximal activity retained Bacillus sp. (in: Bacteria)