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Literature summary extracted from

  • Kashyap, D.R.; Chandra, S.; Kaul, A.; Tewari, R.
    Production, purification and characterization of pectinase from a Bacillus sp. DT7 (2000), World J. Microbiol. Biotechnol., 16, 277-282.
    View publication on PubMed

Application

EC Number Application Comment Organism
4.2.2.10 additional information tremendous applications in textile industry, plant tissue maceration, and fruit juice waste water treatment Bacillus sp. (in: Bacteria)

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.2.2.10 extracellular
-
 Bacillus sp. (in: Bacteria)
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.2.10 2-mercaptoethanol activates 1.5fold at 100 mM Bacillus sp. (in: Bacteria)
4.2.2.10 CaCl2 activates the purified enzyme by 50% at 1-100 mM, activates at 0.005% in the culture medium, activation is lowered in presence of MgSO4 Bacillus sp. (in: Bacteria)
4.2.2.10 MgSO4 no effect on purified enzyme, activates at 0.005% in the culture medium, activation is lowered in presence of CaCl2 Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.2.10 106000
-
 x * 106000, SDS-PAGE Bacillus sp. (in: Bacteria)

Organism

EC Number Organism UniProt Comment Textmining
4.2.2.10 Bacillus sp. (in: Bacteria)
-
-
-
4.2.2.10 Bacillus sp. (in: Bacteria) DT7
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.2.10 131.8fold Bacillus sp. (in: Bacteria)

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.2.10 additional information the bacterium is a soil isolate, optimization of culture medium conditions, overview Bacillus sp. (in: Bacteria)
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.2.10 additional information
-
 activity at different culture conditions, U/ml Bacillus sp. (in: Bacteria)
4.2.2.10 1433
-
 purified enzyme Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.2.10 pectin
-
 Bacillus sp. (in: Bacteria) ?
-
 ?
4.2.2.10 pectin
-
 Bacillus sp. (in: Bacteria) DT7 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.2.10 ? x * 106000, SDS-PAGE Bacillus sp. (in: Bacteria)

Synonyms

EC Number Synonyms Comment Organism
4.2.2.10 pectin lyase
-
 Bacillus sp. (in: Bacteria)
4.2.2.10 pectinase
-
 Bacillus sp. (in: Bacteria)

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.2.10 60
-
-
 Bacillus sp. (in: Bacteria)

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4.2.2.10 40 60
-
 Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.2.10 60
-
 1 h, partially stable Bacillus sp. (in: Bacteria)

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.2.10 8
-
-
 Bacillus sp. (in: Bacteria)

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.2.2.10 6 9 enzyme is optimally stable at pH 7.5-8.5, 50% activity at neutral pH, complete loss of activity occurs below pH 6.0 and above pH 9.0 Bacillus sp. (in: Bacteria)