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Literature summary extracted from

  • Giabbai, B.; Degano, M.
    Crystal structure to 1.7 ANG of the Escherichia coli pyrimidine nucleoside hydrolase YeiK, a novel candidate for cancer gene therapy (2004), Structure, 12, 739-749.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.2.8
-
 Escherichia coli

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.2.8 hanging drop vapor diffusion method Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
3.2.2.8 H239A dramatic increase in Km for uridine, unchanged kcat Escherichia coli
3.2.2.8 H82N small increase in Km, increase in kcat Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.2.8 0.128
-
 5-fluorouridine pH 7.3, 37°C Escherichia coli
3.2.2.8 0.142
-
 uridine pH 7.3, 37°C Escherichia coli
3.2.2.8 0.186
-
 5-Bromouridine pH 7.3, 37°C Escherichia coli
3.2.2.8 0.22
-
 5-iodouridine pH 7.3, 37°C Escherichia coli
3.2.2.8 0.329
-
 5-methyluridine pH 7.3, 37°C Escherichia coli
3.2.2.8 0.345
-
 uridine pH 7.3, 37°C, H82N mutant Escherichia coli
3.2.2.8 0.532
-
 cytidine pH 7.3, 37°C Escherichia coli
3.2.2.8 1.9
-
 uridine pH 7.3, 37°C, H239A mutant Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.2.8 33000
-
 homotetramer, 4 * 33000, SDS-PAGE Escherichia coli
3.2.2.8 137000
-
 calculated from amino acid sequence Escherichia coli
3.2.2.8 143000
-
 gel filtration Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
3.2.2.8 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.2.8
-
 Escherichia coli

Reaction

EC Number Reaction Comment Organism Reaction ID
3.2.2.8 a pyrimidine nucleoside + H2O = D-ribose + a pyrimidine base mechanism Escherichia coli
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.2.8 5-bromouridine + H2O
-
 Escherichia coli 5-bromouracil + D-ribose
-
 ?
3.2.2.8 5-fluorouridine + H2O
-
 Escherichia coli 5-fluorouracil + D-ribose
-
 ?
3.2.2.8 5-iodouridine + H2O
-
 Escherichia coli 5-iodouracil + D-ribose
-
 ?
3.2.2.8 5-methyluridine + H2O
-
 Escherichia coli 5-methyluracil + D-ribose
-
 ?
3.2.2.8 cytidine + H2O
-
 Escherichia coli cytosine + D-ribose
-
 ?
3.2.2.8 additional information no substrate: deazouridine Escherichia coli ?
-
 ?
3.2.2.8 uridine + H2O
-
 Escherichia coli uracil + D-ribose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.2.8 tetramer homotetramer, 4 * 33000, SDS-PAGE Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
3.2.2.8 YeiK
-
 Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.2.8 4.4
-
 uridine pH 7.3, 37°C, H239A mutant Escherichia coli
3.2.2.8 4.7
-
 uridine pH 7.3, 37°C Escherichia coli
3.2.2.8 11.6
-
 cytidine pH 7.3, 37°C Escherichia coli
3.2.2.8 14.7
-
 5-fluorouridine pH 7.3, 37°C Escherichia coli
3.2.2.8 15.5
-
 uridine pH 7.3, 37°C, H82N mutant Escherichia coli
3.2.2.8 25.5
-
 5-methyluridine pH 7.3, 37°C Escherichia coli
3.2.2.8 30.8
-
 5-Bromouridine pH 7.3, 37°C Escherichia coli
3.2.2.8 42.7
-
 5-iodouridine pH 7.3, 37°C Escherichia coli