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Literature summary extracted from

  • Bonin, I.; Martins, B.M.; Purvanov, V.; Fetzner, S.; Huber, R.; Dobbek, H.
    Active site geometry and substrate recognition of the molybdenum hydroxylase quinoline 2-oxidoreductase (2004), Structure, 12, 1425-1435.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.3.99.17
-
 Pseudomonas putida

Protein Variants

EC Number Protein Variants Comment Organism
1.3.99.17 E743D about twofold increase in KM-value for quinoline, 150fold reduction of kcat-value Pseudomonas putida
1.3.99.17 E743V about twofold increase in KM-value for quinoline, drastic reduction of kcat-value Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.99.17 0.12
-
 Quinoline wild-type Pseudomonas putida
1.3.99.17 0.25
-
 Quinoline mutant E743D Pseudomonas putida
1.3.99.17 0.26
-
 Quinoline mutant E743V Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
1.3.99.17 Pseudomonas putida
-
 recombinant enzyme
-
1.3.99.17 Pseudomonas putida 86
-
 recombinant enzyme
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.3.99.17 quinoline + acceptor + H2O = quinolin-2(1H)-one + reduced acceptor putative substrate binding mode Pseudomonas putida
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.99.17 quinoline + H2O + iodonitrotetrazolium chloride
-
 Pseudomonas putida isoquinolin-1-one + reduced iodonitrotetrazolium chloride
-
 ?
1.3.99.17 quinoline + H2O + iodonitrotetrazolium chloride
-
 Pseudomonas putida 86 isoquinolin-1-one + reduced iodonitrotetrazolium chloride
-
 ?

Subunits

EC Number Subunits Comment Organism
1.3.99.17 hexamer 2 * iron-sulfur protein, S subunit, + 2 * flavoprotein, M subunit, + 2 * L subunit carrying the molybdopterin cytosine dinucleotide, crystallization data Pseudomonas putida

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.3.99.17 0.22
-
 Quinoline mutant E743V Pseudomonas putida
1.3.99.17 0.56
-
 Quinoline mutant E743D Pseudomonas putida
1.3.99.17 85.4
-
 Quinoline wild-type Pseudomonas putida