EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.36 | expression of a truncated enzyme, comprising residues Met1-Asp319 of the dehydrogenase region, lacking the hydratase-2 region, in Escherichia coli | Rattus norvegicus |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
1.1.1.36 | selenomethionine-labeled recombinant truncated enzyme, X-ray diffraction structure determination and analysis at 2.38 A resolution | Rattus norvegicus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.1.1.36 | peroxisome | enzyme contains a C-terminal peroxisomal targeting signal sequence | Rattus norvegicus | 5777 | - |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.36 | (R)-3-hydroxybutyryl-CoA | Rattus norvegicus | dehydration, multifunctional type 2 enzyme | trans-2-butenoyl-CoA + H2O | - |
? | |
1.1.1.36 | (R)-3-hydroxybutyryl-CoA + NAD+ | Rattus norvegicus | oxidation, multifunctional type 2 enzyme | acetoacetyl-CoA + NADH | - |
? | |
1.1.1.36 | additional information | Rattus norvegicus | a point mutation in the dehydrogenase region of the enzyme causes peroxisomal disease leading to severe abnormalities and an early death | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.36 | Rattus norvegicus | - |
multifunctional enzyme type 2, i.e. MFE-2 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.36 | recombinant truncated enzyme from Escherichia coli, to homogeneity | Rattus norvegicus |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
1.1.1.36 | (R)-3-hydroxyacyl-CoA + NADP+ = 3-oxoacyl-CoA + NADPH + H+ | catalytic site structure and catalytic triad Ser151, Tyr164, and Lys168 | Rattus norvegicus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.36 | (R)-3-hydroxybutyryl-CoA | dehydration, multifunctional type 2 enzyme | Rattus norvegicus | trans-2-butenoyl-CoA + H2O | - |
? | |
1.1.1.36 | (R)-3-hydroxybutyryl-CoA + NAD+ | oxidation, multifunctional type 2 enzyme | Rattus norvegicus | acetoacetyl-CoA + NADH | - |
? | |
1.1.1.36 | additional information | a point mutation in the dehydrogenase region of the enzyme causes peroxisomal disease leading to severe abnormalities and an early death | Rattus norvegicus | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.1.36 | dimer | crystal structure, enzyme shows a alpha/beta short chain alcohol dehydrogenase/reductase, i.e. SDR, fold | Rattus norvegicus |
1.1.1.36 | More | the recombinant truncated and selenomethionine-labeled enzyme has a molecular weight of 34602 Da as determined by mass spectrometry | Rattus norvegicus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.36 | (3R)-hydroxyacyl-CoA dehydrogenase | - |
Rattus norvegicus |
1.1.1.36 | MFE-2 | - |
Rattus norvegicus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.36 | NAD+ | binding mode and conformation of NAD+ bound to the enzyme derived from the crystal structure, overview | Rattus norvegicus |