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Literature summary extracted from

  • Thomas, B.C.; Chamberlain, J.; Engelke, D.R.; Gegenheimer, P.
    Evidence for an RNA-based catalytic mechanism in eukaryotic nuclear ribonuclease P (2000), RNA, 6, 554-562.
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
3.1.26.5 additional information construction of a pre-tRNA substrate in which the pro-Rp nonbridging oxygen of the scissile bond is replaced by sulfur Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.26.5 phosphorothionate inhibition of activity due to phosphothionate substitution at the pro-Rp or pro-Sp nonbridging position of the scissile bond in tRNA, activity cannot be recovered by Mn2+ Saccharomyces cerevisiae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.26.5 nucleus
-
 Saccharomyces cerevisiae 5634
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.26.5 Mg2+ dependent on, binds to the pro-Rp nonbridging oxygen of the scissile bond, coordination Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.26.5 tRNA precursor + H2O Saccharomyces cerevisiae enzyme is responsible for removing the 5'-leader segment of precursor tRNA mature tRNA + 5'-terminal oligonucleotide
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.26.5 Saccharomyces cerevisiae
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.26.5 ribonucleoprotein
-
 Saccharomyces cerevisiae

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.26.5 partially Saccharomyces cerevisiae

Reaction

EC Number Reaction Comment Organism Reaction ID
3.1.26.5 endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor an RNA-containing enzyme, essential for tRNA processing, generates 5'-termini or mature tRNA molecules, RNA-based catalytic mechanism Saccharomyces cerevisiae
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.26.5 additional information no activity with substrates in which the pro-Rp or pro-Sp nonbridging oxygen of the scissile bond is replaced by sulfur, substitution via phosphothionate Saccharomyces cerevisiae ?
-
 ?
3.1.26.5 tRNA precursor + H2O enzyme is responsible for removing the 5'-leader segment of precursor tRNA Saccharomyces cerevisiae mature tRNA + 5'-terminal oligonucleotide
-
 ?
3.1.26.5 tRNAPhe (A+1) precursor + H2O yeast tRNA substrate from in vitro transcription, structure Saccharomyces cerevisiae mature tRNAPhe + 5'-terminal oligonucleotide 5'-terminal oligonucleotide with 3'-hydroxyl and 5'-phosphoryl, product and cleavage site determination ?
3.1.26.5 tRNAPhe (G+1) precursor + H2O yeast tRNA substrate from in vitro transcription, structure Saccharomyces cerevisiae mature tRNAPhe + 5'-terminal oligonucleotide 5'-terminal oligonucleotide with 3'-hydroxyl and 5'-phosphoryl, product and cleavage site determination ?

Subunits

EC Number Subunits Comment Organism
3.1.26.5 More enzyme is a ribonucleoprotein consisting of multiple protein components and a single RNA species Saccharomyces cerevisiae

Synonyms

EC Number Synonyms Comment Organism
3.1.26.5 RNase P
-
 Saccharomyces cerevisiae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.26.5 30
-
 assay at Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.26.5 8
-
 assay at Saccharomyces cerevisiae