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Literature summary extracted from

  • Kumar, P.; Kothari, H.; Singh, N.
    Overexpression in Escherichia coli and purification of pteridine reductase (PTR1) from a clinical isolate of Leishmania donovani (2004), Protein Expr. Purif., 38, 228-236.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.5.1.33 medicine enzyme is an important chemotherapeutic target for drugs against Leishmania Leishmania donovani

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.1.33 overexpression of the N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) Leishmania donovani

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.1.33 29000
-
 4 * 33500, recombinant enzyme, SDS-PAGE, 4 * 29000, native enzyme, SDS-PAGE Leishmania donovani
1.5.1.33 33500
-
 4 * 33500, recombinant enzyme, SDS-PAGE, 4 * 29000, native enzyme, SDS-PAGE Leishmania donovani
1.5.1.33 134000
-
 recombinant enzyme, gel filtration Leishmania donovani

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.1.33 additional information Leishmania donovani enzyme is associated with folate metabolism, responsible for salvage of pterins and reduction of folates ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.33 Leishmania donovani Q6QDB5 clinical isolate
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.33 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3)by nickel affinity chromatography Leishmania donovani

Reaction

EC Number Reaction Comment Organism Reaction ID
1.5.1.33 5,6,7,8-tetrahydrobiopterin + 2 NADP+ = biopterin + 2 NADPH + 2 H+ conserved residue Tyr194 forms hydrogen bonds with the nucleophilic center of the substrate and stabilizes the transition state, conserved residue Lys198 lowers the pKa of the tyrosine, helps to stabilize the transition state, and may function as proton source for the tyrosine Leishmania donovani
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.33 additional information enzyme is associated with folate metabolism, responsible for salvage of pterins and reduction of folates Leishmania donovani ?
-
 ?
1.5.1.33 additional information NADPH binds first, the enzyme-NADPH complex binds the substrate Leishmania donovani ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.5.1.33 tetramer 4 * 33500, recombinant enzyme, SDS-PAGE, 4 * 29000, native enzyme, SDS-PAGE Leishmania donovani

Synonyms

EC Number Synonyms Comment Organism
1.5.1.33 pteridine reductase 1
-
 Leishmania donovani
1.5.1.33 PTR1
-
 Leishmania donovani

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.33 NADPH binding site is located at residues Gly13 to Arg39, recognition motif GXXXRXG Leishmania donovani