Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Poletto, S.S.; da Fonseca, I.O.; de Carvalho, L.P.; Basso, L.A.; Santos, D.S.
    Selection of an Escherichia coli host that expresses mutant forms of Mycobacterium tuberculosis 2-trans enoyl-ACP(CoA) reductase and 3-ketoacyl-ACP(CoA) reductase enzymes (2004), Protein Expr. Purif., 34, 118-125.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.3.1.44 medicine enzyme is a target for drugs in treatment of tuberculosis Mycobacterium tuberculosis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.100 selection of an Escherichia coli, BL21(DE3)NH host that stably expresses mutant forms of 3-ketoacyl-ACP(CoA) reductase Mycobacterium tuberculosis
1.3.1.44 gene inhA, overexpression of stable mutant enzymes in Escherichia coli strain BL21(DE3) Mycobacterium tuberculosis

Protein Variants

EC Number Protein Variants Comment Organism
1.3.1.38 K165A normal expression in Escherichia coli Mycobacterium tuberculosis
1.3.1.38 K165Q normal expression in Escherichia coli Mycobacterium tuberculosis
1.3.1.38 Y158F expression in Escherichia coli sub-population BL21(DE3)NH Mycobacterium tuberculosis
1.3.1.44 K165A site-directed mutagenesis, mutation of putative catalytic residue Mycobacterium tuberculosis
1.3.1.44 K165Q site-directed mutagenesis, mutation of putative catalytic residue Mycobacterium tuberculosis
1.3.1.44 additional information construction of stable mutants for optimization of expression of enzyme in Escherichia coli, absolutely dependent on IPTG, phenotype alterations, overview Mycobacterium tuberculosis
1.3.1.44 Y158F site-directed mutagenesis, mutation of putative catalytic residue, mutant can only be stably expressed in Escherichia coli strain BL21(DE3) if altered to a soluble enzyme form dependent on IPTG induction Mycobacterium tuberculosis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.1.44 isoniazid drug to treat tuberculosis Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.100 Mycobacterium tuberculosis
-
-
-
1.3.1.38 Mycobacterium tuberculosis
-
-
-
1.3.1.44 Mycobacterium tuberculosis
-
 enzyme is a component of fatty acid synthase type II
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.38 acyl-CoA + NADP+
-
 Mycobacterium tuberculosis trans-2,3-dehydroacyl-CoA + NADPH
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.100 3-ketoacyl-ACP(CoA) reductase
-
 Mycobacterium tuberculosis
1.3.1.38 2-trans enoyl-ACP(CoA) reductase
-
 Mycobacterium tuberculosis
1.3.1.38 InhA
-
 Mycobacterium tuberculosis
1.3.1.44 ENR
-
 Mycobacterium tuberculosis
1.3.1.44 More enzyme belongs to the short-chain dehydrogenase/reductase family, enzyme is a component of fatty acid synthase type II Mycobacterium tuberculosis
1.3.1.44 trans-2-enoyl-ACP(CoA) reductase
-
 Mycobacterium tuberculosis

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.38 NADP+
-
 Mycobacterium tuberculosis
1.3.1.44 NADH dependent on Mycobacterium tuberculosis