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Literature summary extracted from

  • Huang, C.Y.; Chao, Y.P.; Yang, Y.S.
    Purification of industrial hydantoinase in one chromatographic step without affinity tag (2003), Protein Expr. Purif., 30, 134-139.
    View publication on PubMed

Organism

EC Number Organism UniProt Comment Textmining
3.5.2.2 Agrobacterium tumefaciens
-
 NRRL B11291
-
3.5.2.2 Agrobacterium tumefaciens NRRL B11291
-
 NRRL B11291
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.2.2 recombinant enzyme from Escherichia coli, purification in one chromatographic step without affinity tag Agrobacterium tumefaciens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.2.2 0.0425
-
-
 Agrobacterium tumefaciens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.2.2 5-leucinyl-hydantoin + H2O
-
 Agrobacterium tumefaciens ?
-
 ?
3.5.2.2 5-leucinyl-hydantoin + H2O
-
 Agrobacterium tumefaciens NRRL B11291 ?
-
 ?
3.5.2.2 dihydrouracil + H2O
-
 Agrobacterium tumefaciens 3-ureidopropionate
-
 ?
3.5.2.2 dihydrouracil + H2O
-
 Agrobacterium tumefaciens NRRL B11291 3-ureidopropionate
-
 ?
3.5.2.2 hydantoin + H2O
-
 Agrobacterium tumefaciens hydantoic acid
-
 ?
3.5.2.2 hydantoin + H2O
-
 Agrobacterium tumefaciens NRRL B11291 hydantoic acid
-
 ?
3.5.2.2 phthalimide + H2O low activity Agrobacterium tumefaciens ?
-
 ?
3.5.2.2 phthalimide + H2O low activity Agrobacterium tumefaciens NRRL B11291 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.2.2 hydantoinase
-
 Agrobacterium tumefaciens