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Literature summary extracted from

  • Chilaka, F.C.; Okeke, C.; Adaikpoh, E.
    Ligand-induced thermal stability in beta-galactosidase from the seeds of the black bean, Kerstingiella geocarpa (2002), Process Biochem., 38, 143-149.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.23 galactose competitive Kerstingiella geocarpa
3.2.1.23 glucose competitive at low concentrations, uncompetitive at high concentrations Kerstingiella geocarpa
3.2.1.23 lactose competitive Kerstingiella geocarpa

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.23 Kerstingiella geocarpa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.23
-
 Kerstingiella geocarpa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.23 seed
-
 Kerstingiella geocarpa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.23 0.9
-
-
 Kerstingiella geocarpa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.23 p-nitrophenyl beta-D-galactopyranoside + H2O
-
 Kerstingiella geocarpa p-nitrophenol + beta-D-galactose
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.23 55
-
-
 Kerstingiella geocarpa

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.23 40 65 40°C: about 45% of maximal activity, 65°C: about 45% of maximal activity Kerstingiella geocarpa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.23 additional information
-
 galactose protects against irreversible thermoinactivation Kerstingiella geocarpa
3.2.1.23 60
-
 2 h, no inactivation in presence of 100 mM galactose Kerstingiella geocarpa
3.2.1.23 80
-
 1 h, complete inactivation. 47% of the activity regenerates within 6 h at 25°C Kerstingiella geocarpa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.23 4.4
-
-
 Kerstingiella geocarpa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.23 3.8 5 pH 3.8: about 50% of maximal activity, pH 5.0: about 55% of maximal activity Kerstingiella geocarpa

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.2.1.23 26
-
 galactose
-
 Kerstingiella geocarpa
3.2.1.23 49
-
 glucose
-
 Kerstingiella geocarpa
3.2.1.23 59.5
-
 lactose
-
 Kerstingiella geocarpa