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Literature summary extracted from
- Pectate lyase A, an enzymatic subunit of the Clostridium cellulovorans cellulosome (2001), Proc. Natl. Acad. Sci. USA, 27, 4125-4129.
No PubMed abstract available
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 4.2.2.22 | - |
Clostridium cellulovorans |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.2.2.22 | CaCl2 | 1 mM reduces activity to less than that without addition of CaCl2 | Clostridium cellulovorans |  |
| 4.2.2.22 | EDTA | 1 mM | Clostridium cellulovorans | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 4.2.2.22 | extracellular | - |
Clostridium cellulovorans | - |
- |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.2.2.22 | CaCl2 | optimal activity at 0.05-0.5 mM | Clostridium cellulovorans | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 4.2.2.22 | 42000 | - |
x * 42000, SDS-PAGE | Clostridium cellulovorans |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.2.22 | polygalacturonic acid | Clostridium cellulovorans | pectate lyase A is a subunit of the extracellular multienzyme complex, called the cellulosome, which is involved in plant cell wall degradation | trigalacturonic acid + digalacturonic acid | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.2.2.22 | Clostridium cellulovorans | Q9AQF3 | ATCC 35296 | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.2.2.22 | recombinant enzyme | Clostridium cellulovorans |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.2.22 | polygalacturonic acid | - |
Clostridium cellulovorans | trigalacturonic acid + digalacturonic acid | - |
? | |
| 4.2.2.22 | polygalacturonic acid | pectate lyase A is a subunit of the extracellular multienzyme complex, called the cellulosome, which is involved in plant cell wall degradation | Clostridium cellulovorans | trigalacturonic acid + digalacturonic acid | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 4.2.2.22 | ? | x * 42000, SDS-PAGE | Clostridium cellulovorans |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 4.2.2.22 | pectate lyase A | is a subunit of the extracellular multienzyme complex, called the cellulosome | Clostridium cellulovorans |
| 4.2.2.22 | PelA | - |
Clostridium cellulovorans |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.2.2.22 | 50 | - |
- |
Clostridium cellulovorans |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.2.2.22 | 40 | 60 | more than 50% of maximal activity at 40°C and at 60°C | Clostridium cellulovorans |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.2.2.22 | 8 | - |
- |
Clostridium cellulovorans |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.2.2.22 | 7 | 8 | pH 7.0: less than 30% of maximal activity, pH 8.0: optimum | Clostridium cellulovorans |
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