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Literature summary extracted from

  • Xie, D.Y.; Jackson, L.A.; Cooper, J.D.; Ferreira, D.; Paiva, N.L.
    Molecular and biochemical analysis of two cDNA clones encoding dihydroflavonol-4-reductase from Medicago truncatula (2004), Plant Physiol., 134, 979-994.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.219 isozyme MtDFR1, from a young seed cDNA library, functional expression in Escherichia coli Medicago truncatula
1.1.1.219 isozyme MtDFR2, from a young seed cDNA library, functional expression in Escherichia coli Medicago truncatula

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.219 additional information overexpression of the isozyme in transgenic Nicotiana tabacum cv. Xanthi under control of the CaMV35S promotor does not lead to increased anthocyanin production in the flowers Medicago truncatula
1.1.1.219 additional information overexpression of the isozyme in transgenic Nicotiana tabacum cv. Xanthi under control of the CaMV35S promotor leads to increased anthocyanin production in the flowers Medicago truncatula

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.219 additional information Medicago truncatula key enzyme in flux control in biosynthetic branched pathways leading to anthocyanins and condensed tannins ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.219 Medicago truncatula Q6TQT0 isozyme MtDFR2; cv. Jemalong, 2 isozymes MtDFR1 and MtDFR2
-
1.1.1.219 Medicago truncatula Q6TQT1 isozyme MtDFR1; cv. Jemalong, 2 isozymes MtDFR1 and MtDFR2
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.219 flower
-
 Medicago truncatula
-
1.1.1.219 leaf
-
 Medicago truncatula
-
1.1.1.219 additional information developmental gene expression pattern Medicago truncatula
-
1.1.1.219 seed
-
 Medicago truncatula
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.219 (+)-2,3-dihydrokaempferol + NADPH
-
 Medicago truncatula cis-3,4-leucopelargonidin + NADP+
-
 ?
1.1.1.219 (+)-2,3-dihydrokaempferol + NADPH low activity Medicago truncatula cis-3,4-leucopelargonidin + NADP+
-
 ?
1.1.1.219 (+/-)-fustin + NADPH preference for (-)-isomer Medicago truncatula ?
-
 ?
1.1.1.219 (-)-fustin + NADPH stereospecific for (-)-isomer Medicago truncatula ?
-
 ?
1.1.1.219 2,3-dihydromyricetin + NADPH low activity Medicago truncatula cis-3,4-leucodelphinidin + NADP+
-
 ?
1.1.1.219 2,3-dihydroquercetin + NADPH i.e. (+)-taxifolin, stereospecific for (+)-isomer Medicago truncatula cis-3,4-leucocyanidin + NADP+
-
 ?
1.1.1.219 2,3-dihydrorobinetin + NADPH
-
 Medicago truncatula ?
-
 ?
1.1.1.219 additional information key enzyme in flux control in biosynthetic branched pathways leading to anthocyanins and condensed tannins Medicago truncatula ?
-
 ?
1.1.1.219 additional information stereospecific enzyme, no activity with substances lacking the the hydroxyl group at the 3-position or with a double bond present between C2 and C3, e.g. quercetin, apigenin, eriodictyol, and kaempferol Medicago truncatula ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.219 DFR
-
 Medicago truncatula
1.1.1.219 dihydroflavonol 4-reductase
-
 Medicago truncatula
1.1.1.219 dihydroflavonol-4-reductase
-
 Medicago truncatula

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.219 30
-
 assay at Medicago truncatula

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.219 6.2
-
-
 Medicago truncatula
1.1.1.219 7
-
-
 Medicago truncatula

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.219 NADPH
-
 Medicago truncatula