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Literature summary extracted from

  • Cheong, N.E.; Choi, Y.O.; Lee, K.O.; Kim, W.Y.; Jung, B.G.; Chi, Y.H.; Jeong, J.S.; Kim, K.; Cho, M.J.; Lee, S.Y.
    Molecular cloning, expression, and functional characterization of a 2Cys-peroxiredoxin in Chinese cabbage (1999), Plant Mol. Biol., 40, 825-834.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.11.1.24 expression of N-terminally truncated enzyme in Escherichia coli, DELTA66-273 Brassica rapa

Protein Variants

EC Number Protein Variants Comment Organism
1.11.1.24 DELTA66-273 the mutant enzyme prevents the inactivation of glutamine synthetase and the DNA cleavage in the metal-catalyzed oxidation system. In the yeast thioredoxin system containing thioredoxin reductase, thioredoxin, and NADPH, the DELTAC2C-Prx exhibits peroxidase activity on H2O2 Brassica rapa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.11.1.24 22000
-
 1 * 22000, mutant enzyme DELTA66-273 migrates as a monomer under reducing conditions Brassica rapa
1.11.1.24 22000
-
 2 * 22000, mutant enzyme DELTA66-273 migrates as a dimer under non-reducing SDS-PAGE Brassica rapa

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.24 Brassica rapa Q9SQJ4 subsp. pekinensis
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.11.1.24 leaf the gene is predominantly expressed in leaf tissue of seedlings Brassica rapa
-
1.11.1.24 additional information the mRNA is generally expressed in most tissues of mature plant, except root Brassica rapa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.24 NADPH + H2O2
-
 Brassica rapa NADP+ + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.11.1.24 dimer 2 * 22000, mutant enzyme DELTA66-273 migrates as a dimer under non-reducing SDS-PAGE Brassica rapa
1.11.1.24 monomer 1 * 22000, mutant enzyme DELTA66-273 migrates as a monomer under reducing conditions Brassica rapa

Synonyms

EC Number Synonyms Comment Organism
1.11.1.24 2Cys-peroxiredoxin
-
 Brassica rapa