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Literature summary extracted from

  • Xiao, Y.Z.; Chen, Q.; Hang, J.; Shi, Y.Y.; Wu, J.; Hong, Y.Z.; Wang, Y.P.
    Selective induction, purification and characterization of a laccase isozyme from the basidiomycete Trametes sp. AH28-2 (2004), Mycologia, 96, 26-35.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.10.3.2 0.028
-
 syringaldazine pH 4.5 Trametes sp.
1.10.3.2 0.11
-
 2,6-dimethoxyphenol pH 4.5 Trametes sp.
1.10.3.2 0.177
-
 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) pH 4.5 Trametes sp.
1.10.3.2 1.249
-
 guaiacol pH 4.5 Trametes sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.10.3.2 copper four ions per molecule Trametes sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.10.3.2 71450
-
 1 * 74000, SDS-PAGE, 1 * 71450, mass spectrometry Trametes sp.
1.10.3.2 72000
-
 gel filtration Trametes sp.
1.10.3.2 74000
-
 1 * 74000, SDS-PAGE, 1 * 71450, mass spectrometry Trametes sp.

Organism

EC Number Organism UniProt Comment Textmining
1.10.3.2 Trametes sp.
-
 induction in cellobiose-based culture medium, selective induction of isozyme B by 3,5-dihydroxytoluene
-
1.10.3.2 Trametes sp. AH28-2
-
 induction in cellobiose-based culture medium, selective induction of isozyme B by 3,5-dihydroxytoluene
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.10.3.2 glycoprotein about 25% carbohydrate Trametes sp.

Purification (Commentary)

EC Number Purification (Comment) Organism
1.10.3.2
-
 Trametes sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.10.3.2 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
-
 Trametes sp. ? + H2O
-
 ?
1.10.3.2 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
-
 Trametes sp. AH28-2 ? + H2O
-
 ?
1.10.3.2 2,6-dimethoxyphenol + O2
-
 Trametes sp. ? + H2O
-
 ?
1.10.3.2 2,6-dimethoxyphenol + O2
-
 Trametes sp. AH28-2 ? + H2O
-
 ?
1.10.3.2 guaiacol + O2
-
 Trametes sp. ? + H2O
-
 ?
1.10.3.2 guaiacol + O2
-
 Trametes sp. AH28-2 ? + H2O
-
 ?
1.10.3.2 syringaldazine + O2
-
 Trametes sp. ?
-
 ?
1.10.3.2 syringaldazine + O2
-
 Trametes sp. AH28-2 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.10.3.2 monomer 1 * 74000, SDS-PAGE, 1 * 71450, mass spectrometry Trametes sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.10.3.2 45
-
 substrate guaiacol Trametes sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.10.3.2 60
-
 14 min, 50% residual activity Trametes sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.10.3.2 140
-
 guaiacol pH 4.5 Trametes sp.
1.10.3.2 189
-
 2,6-dimethoxyphenol pH 4.5 Trametes sp.
1.10.3.2 600
-
 syringaldazine pH 4.5 Trametes sp.
1.10.3.2 2277
-
 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) pH 4.5 Trametes sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.10.3.2 4.7
-
 substrate guaiacol Trametes sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.10.3.2 3.5 7.5
-
 Trametes sp.

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.10.3.2 Trametes sp. isoelectric focusing
-
 4