Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Martinez, M.L.; Martelotto, L.; Cabada, M.O.
    Purification and biological characterization of N-acetyl-beta-D-glucosaminidase from Bufo arenarum spermatozoa (2000), Mol. Reprod. Dev., 57, 194-203.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.52 N-acetyl-beta-D-galactosamine competitive Rhinella arenarum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.52 0.59
-
 p-nitrophenyl-beta-D-N-acetylglucosaminide
-
 Rhinella arenarum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.52 membrane
-
 Rhinella arenarum 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.52 45000
-
 1 * 45000, SDS-PAGE Rhinella arenarum
3.2.1.52 45000 50000 gel filtration Rhinella arenarum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.52 additional information Rhinella arenarum major sperm glycosidase, plays an important role in toad fertilization ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.52 Rhinella arenarum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.52
-
 Rhinella arenarum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.52 sperm
-
 Rhinella arenarum
-

Storage Stability

EC Number Storage Stability Organism
3.2.1.52 0-4°C, stable for several days without appreciable loss of activity Rhinella arenarum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.52 additional information major sperm glycosidase, plays an important role in toad fertilization Rhinella arenarum ?
-
 ?
3.2.1.52 p-nitrophenyl-beta-D-N-acetylglucosaminide + H2O
-
 Rhinella arenarum p-nitrophenol + beta-D-N-acetylglucosamine
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.52 monomer 1 * 45000, SDS-PAGE Rhinella arenarum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.52 55
-
 hydrolysis of p-nitrophenyl-beta-D-N-acetylglucosaminide Rhinella arenarum

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.52 30 60 30°C: about 50% of maximal activity, 60°C: about 90% of maximal activity, 80°C: about 30% of maximal activity, hydrolysis of p-nitrophenyl-beta-D-N-acetylglucosaminide Rhinella arenarum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.52 3.5
-
 and a small peak at pH 5.0 Rhinella arenarum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.52 3 6.5 pH 3.0: 95% of maximal activity, pH 5.0: about 65% of maximal activity, pH 4.5 and pH 6.5: about 45% of maximal activity, pH 7.0: about 25% of maximal activity Rhinella arenarum

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.2.1.52 3.2
-
 N-acetyl-beta-D-galactosamine
-
 Rhinella arenarum