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Literature summary extracted from
- Purification and characterization of two isozymes of polygalacturonase from Botrytis cinerea. Effect of calcium ions on polygalacturonase activity (2002), Microbiol. Res., 157, 183-189.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.15 | CaCl2 | probably by calcium chelation of polygalacturonic acid | Botrytis cinerea |  |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.2.1.67 | extracellular | - |
Botrytis cinerea | - |
- |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.15 | 52000 | - |
x * 52000, SDS-PAGE | Botrytis cinerea |
| 3.2.1.67 | 63000 | - |
gel filtration | Botrytis cinerea |
| 3.2.1.67 | 65000 | - |
1 * 65000, SDS-PAGE | Botrytis cinerea |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.15 | Botrytis cinerea | - |
- |
- |
| 3.2.1.67 | Botrytis cinerea | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.15 | PG II | Botrytis cinerea |
| 3.2.1.67 | - |
Botrytis cinerea |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.2.1.15 | cell culture | liquid culture | Botrytis cinerea | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.15 | 1.25 | - |
- |
Botrytis cinerea |
| 3.2.1.67 | 0.6 | - |
pH 4.5, 30°C | Botrytis cinerea |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.15 | polygalacturonate + H2O | - |
Botrytis cinerea | oligogalacturonates | - |
? | |
| 3.2.1.67 | (1,4-alpha-D-galacturonide)n + H2O | - |
Botrytis cinerea | D-galacturonic acid | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.2.1.15 | ? | x * 52000, SDS-PAGE | Botrytis cinerea |
| 3.2.1.67 | monomer | 1 * 65000, SDS-PAGE | Botrytis cinerea |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.1.15 | endopolygalacturonase | - |
Botrytis cinerea |
| 3.2.1.15 | PG II | - |
Botrytis cinerea |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.15 | 4.5 | - |
- |
Botrytis cinerea |
| 3.2.1.67 | 4.5 | - |
- |
Botrytis cinerea |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.67 | 3 | 6 | - |
Botrytis cinerea |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 3.2.1.15 | Botrytis cinerea | isoelectric focusing | - |
6.9 |
| 3.2.1.67 | Botrytis cinerea | isoelectric focusing | - |
8 |
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