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Literature summary extracted from
- Escherichia coli ribonuclease II (2001), Methods Enzymol., 342, 309-330.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.13.1 | DNA oligonucleotide with strong duplex structure and 3' single strand | - |
Escherichia coli | |
| 3.1.13.1 | DNA oligonucleotide with strong duplex structure and 5' single strand | - |
Escherichia coli | |
| 3.1.13.1 | DNA oligonucleotide with strong duplex structure, 3' and 5' single strand | - |
Escherichia coli | |
| 3.1.13.1 | DNA oligonucleotide with weak duplex structure and 3' single strand | - |
Escherichia coli | |
| 3.1.13.1 | DNA oligonucleotide with weak duplex structure and 5' single strand | - |
Escherichia coli | |
| 3.1.13.1 | DNA oligonucleotide with weak duplex structure, 3' and 5' single strand | - |
Escherichia coli | |
| 3.1.13.1 | poly(dC) | 19 to 29 monomer units chain lenght | Escherichia coli |  |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.13.1 | 0.0004 | - |
polyadenosine | pH 7.2, 23°C | Escherichia coli |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.13.1 | K+ | required | Escherichia coli | |
| 3.1.13.1 | Mg2+ | required | Escherichia coli | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.13.1 | RNA | Escherichia coli | 3' to 5'direction only | 5'-phosphomononucleotides | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.13.1 | Escherichia coli | - |
- |
- |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.13.1 | poly(A) | - |
Escherichia coli | 5'-AMP | - |
? | |
| 3.1.13.1 | poly(C) | - |
Escherichia coli | 5'-CMP | - |
? | |
| 3.1.13.1 | poly(G) | - |
Escherichia coli | 5'-GMP | - |
? | |
| 3.1.13.1 | poly(U) | - |
Escherichia coli | 5'-UMP | - |
? | |
| 3.1.13.1 | polyadenosine + H2O | - |
Escherichia coli | ? | - |
? | |
| 3.1.13.1 | RNA | 3' to 5'direction only | Escherichia coli | 5'-phosphomononucleotides | - |
? | |
Ki Value [mM]
| EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.13.1 | 0.0005 | - |
polydeoxycytidine | 29 monomer units, pH 7.2, 23°C | Escherichia coli | |
| 3.1.13.1 | 0.0005 | - |
DNA oligonucleotide with weak duplex structure, 3' and 5' single strand | pH 7.2, 23°C | Escherichia coli | |
| 3.1.13.1 | 0.0007 | - |
polydeoxycytidine | 25 monomer units, pH 7.2, 23°C | Escherichia coli | |
| 3.1.13.1 | 0.0008 | - |
DNA oligonucleotide with weak duplex structure and 3' single strand | pH 7.2, 23°C | Escherichia coli | |
| 3.1.13.1 | 0.0014 | - |
DNA oligonucleotide with strong duplex structure, 3' and 5' single strand | pH 7.2, 23°C | Escherichia coli | |
| 3.1.13.1 | 0.0015 | - |
polydeoxycytidine | 23 monomer units, pH 7.2, 23°C | Escherichia coli | |
| 3.1.13.1 | 0.0037 | - |
DNA oligonucleotide with strong duplex structure and 3' single strand | pH 7.2, 23°C | Escherichia coli | |
| 3.1.13.1 | 0.0057 | - |
polydeoxycytidine | 21 monomer units, pH 7.2, 23°C | Escherichia coli | |
| 3.1.13.1 | 0.0148 | - |
polydeoxycytidine | 19 monomer units, pH 7.2, 23°C | Escherichia coli | |
| 3.1.13.1 | 0.0331 | - |
DNA oligonucleotide with strong duplex structure and 5' single strand | pH 7.2, 23°C | Escherichia coli | |
| 3.1.13.1 | 0.0336 | - |
DNA oligonucleotide with weak duplex structure and 5' single strand | pH 7.2, 23°C | Escherichia coli |
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