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Literature summary extracted from

  • Martinez-Ruiz, A.; Garcia-Ortega, L.; Kao, R.; Lacadena, J.; Onaderra, M.; Mancheno, J.M.; Davies, J.; Del Pozo, A.M.; Gavilanes, J.G.
    RNase U2 and alpha-sarcin: a study of relationships (2001), Methods Enzymol., 341, 335-351.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.6.1.20 expression in Pichia pastoris Ustilago sphaerogena

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.6.1.20 12490
-
-
 Ustilago sphaerogena

Organism

EC Number Organism UniProt Comment Textmining
4.6.1.20 Ustilago sphaerogena P00654 ATCC 12421 and CBS 534.71
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.6.1.20 purification of recombinant enzyme using several chromatographic steps, including DEAE-cellulose and affinity chromatography on 2'-5'-ADP-Sepharose Ustilago sphaerogena

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.6.1.20 RNA + H2O
-
 Ustilago sphaerogena 3'-Phosphomononucleotides + 3'-phosphooligonucleotides + 2',3'-cyclic nucleotide phosphates
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.6.1.20 RNase U2
-
 Ustilago sphaerogena

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.6.1.20 5
-
-
 Ustilago sphaerogena

pI Value

EC Number Organism Comment pI Value Maximum pI Value
4.6.1.20 Ustilago sphaerogena
-
 4.8 4.7