Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Parker, K.N.; Chhabra, S.; Lam, D.; Snead, M.A.; Mathur, E.J.; Kelly, R.M.
    beta-Mannosidase from Thermotoga species (2001), Methods Enzymol., 330, 238-246.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.25 3.1
-
 p-nitrophenyl beta-D-mannopyranoside
-
 Thermotoga neapolitana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.25 intracellular
-
 Thermotoga neapolitana 5622
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.25 95000
-
 2 * 95000, SDS-PAGE Thermotoga neapolitana
3.2.1.25 200000
-
-
 Thermotoga neapolitana

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.25 Thermotoga neapolitana
-
-
-
3.2.1.25 Thermotoga neapolitana 5068
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.25
-
 Thermotoga neapolitana

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.25 34
-
-
 Thermotoga neapolitana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.25 p-nitrophenyl beta-D-mannopyranoside + H2O
-
 Thermotoga neapolitana p-nitrophenol + beta-D-mannose
-
 ?
3.2.1.25 p-nitrophenyl beta-D-mannopyranoside + H2O
-
 Thermotoga neapolitana 5068 p-nitrophenol + beta-D-mannose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.25 dimer 2 * 95000, SDS-PAGE Thermotoga neapolitana

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.25 87
-
-
 Thermotoga neapolitana

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.25 85
-
 half-life: 18 h Thermotoga neapolitana
3.2.1.25 90
-
 half-life: 42 min Thermotoga neapolitana
3.2.1.25 98
-
 half-life: 2 min Thermotoga neapolitana

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.25 67.8
-
 p-nitrophenyl beta-D-mannopyranoside
-
 Thermotoga neapolitana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.25 7.7
-
-
 Thermotoga neapolitana

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.25 Thermotoga neapolitana
-
-
 6