BRENDA - Enzyme Database show

Steroid sulfatase activity in the rat ovary, cultured granulosa cells, and a granulosa cell line

Clemens, J.W.; Kabler, H.L.; Sarap, J.L.; Beyer, A.R.; Li, P.K.; Selcer, K.W.; J. Steroid Biochem. Mol. Biol. 75, 245-252 (2000)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.6.2
estrone-3-O-sulfamate
highly potent, specific STS inhibitor, complete inhibition at 0.001 mM
Rattus norvegicus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.6.2
0.072
-
Estrone sulfate
pH 7.5, 37°C, POGS-5 microsomes
Rattus norvegicus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.6.2
microsome
from POGS-5 cell line
Rattus norvegicus
-
-
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.6.2
additional information
Rattus norvegicus
ovarian estrogen synthesis, the relative contribution of STS activity to ovarian steroidogenesis is unclear but may have important physiological and pathophysiological implications
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.6.2
Rattus norvegicus
-
immature female Sprague-Dawley rats
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.6.2
granulosa cell
primary cultures of granulosa cells matured in vitro with follicle-stimulating hormone and testosterone
Rattus norvegicus
-
3.1.6.2
additional information
no STS activity in the immortalized rat granulosa cell line POGRS-1
Rattus norvegicus
-
3.1.6.2
ovary
crude homogenate, from immature female Sprague-Dawley rats treated with gonadotropin in vivo
Rattus norvegicus
-
3.1.6.2
POGS-5 cell
immortalized rat granulosa cell line
Rattus norvegicus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.6.2
dehydroepiandrosterone sulfate + H2O
-
656638
Rattus norvegicus
dehydroepiandrosterone + sulfate
-
-
-
?
3.1.6.2
estrone sulfate + H2O
-
656638
Rattus norvegicus
estrone + sulfate
-
-
-
?
3.1.6.2
additional information
ovarian estrogen synthesis, the relative contribution of STS activity to ovarian steroidogenesis is unclear but may have important physiological and pathophysiological implications
656638
Rattus norvegicus
?
-
-
-
-
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.1.6.2
37
-
assay at
Rattus norvegicus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.6.2
7.5
-
assay at
Rattus norvegicus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.6.2
estrone-3-O-sulfamate
highly potent, specific STS inhibitor, complete inhibition at 0.001 mM
Rattus norvegicus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.6.2
0.072
-
Estrone sulfate
pH 7.5, 37°C, POGS-5 microsomes
Rattus norvegicus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.6.2
microsome
from POGS-5 cell line
Rattus norvegicus
-
-
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.6.2
additional information
Rattus norvegicus
ovarian estrogen synthesis, the relative contribution of STS activity to ovarian steroidogenesis is unclear but may have important physiological and pathophysiological implications
?
-
-
-
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.6.2
granulosa cell
primary cultures of granulosa cells matured in vitro with follicle-stimulating hormone and testosterone
Rattus norvegicus
-
3.1.6.2
additional information
no STS activity in the immortalized rat granulosa cell line POGRS-1
Rattus norvegicus
-
3.1.6.2
ovary
crude homogenate, from immature female Sprague-Dawley rats treated with gonadotropin in vivo
Rattus norvegicus
-
3.1.6.2
POGS-5 cell
immortalized rat granulosa cell line
Rattus norvegicus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.6.2
dehydroepiandrosterone sulfate + H2O
-
656638
Rattus norvegicus
dehydroepiandrosterone + sulfate
-
-
-
?
3.1.6.2
estrone sulfate + H2O
-
656638
Rattus norvegicus
estrone + sulfate
-
-
-
?
3.1.6.2
additional information
ovarian estrogen synthesis, the relative contribution of STS activity to ovarian steroidogenesis is unclear but may have important physiological and pathophysiological implications
656638
Rattus norvegicus
?
-
-
-
-
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.1.6.2
37
-
assay at
Rattus norvegicus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.6.2
7.5
-
assay at
Rattus norvegicus