EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.192 | gene nosE, overexpression in Escherichia coli BL21(DE3) as N-terminally His-tagged enzyme | Nostoc sp. |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.192 | 0.24 | - |
(2S,4S)-5-hydroxyleucine | pH 10.0, 42°C, recombinant enzyme | Nostoc sp. | |
1.1.1.192 | 1.2 | - |
(2S)-5-hydroxy-2-aminovaleric acid | pH 10.0, 42°C, recombinant enzyme | Nostoc sp. |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.192 | Zn2+ | dependent on | Nostoc sp. |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.1.192 | 35000 | - |
x * 35000, about, recombinant enzyme, SDS-PAGE | Nostoc sp. |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.192 | (2S,4S)-5-hydroxyleucine + NAD+ + H2O | Nostoc sp. | enzyme reaction is part of the biosynthesis of (2S,4S)-4-methylproline | (4S)-5-oxo-L-leucine + NADH | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.192 | Nostoc sp. | - |
GSV224, gene nosE | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.192 | recombinant His-tagged enzyme by nickel affinity chromatography | Nostoc sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.192 | (2S)-5-hydroxy-2-aminovaleric acid + NAD+ + H2O | no activity with the (2R)-isomer | Nostoc sp. | (2S)-2-amino-5-oxopentanoate + NADH | - |
? | |
1.1.1.192 | (2S,4S)-5-hydroxyleucine + NAD+ + H2O | - |
Nostoc sp. | (4S)-5-oxo-L-leucine + NADH | - |
r | |
1.1.1.192 | (2S,4S)-5-hydroxyleucine + NAD+ + H2O | enzyme reaction is part of the biosynthesis of (2S,4S)-4-methylproline | Nostoc sp. | (4S)-5-oxo-L-leucine + NADH | - |
r | |
1.1.1.192 | additional information | stereospecific abstraction of the 5pro-S hydrogen | Nostoc sp. | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.1.192 | ? | x * 35000, about, recombinant enzyme, SDS-PAGE | Nostoc sp. |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.192 | long-chain dehydrogenase | - |
Nostoc sp. |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.192 | 42 | - |
- |
Nostoc sp. |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.192 | 10 | - |
- |
Nostoc sp. |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.192 | NAD+ | specific for the A-side of NAD+, no activity with NADP+ | Nostoc sp. |