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Literature summary extracted from

  • von Ossowski, I.; Stahlberg, J.; Koivula, A.; Piens, K.; Becker, D.; Boer, H.; Harle, R.; Harris, M.; Divne, C.; Mahdi, S.; Zhao, Y.; Driguez, H.; Claeyssens, M.; Sinnott, M.L.; Teeri, T.T.
    Engineering the exo-loop of Trichoderma reesei cellobiohydrolase, Cel7A. A comparison with Phanerochaete chrysosporium Cel7D (2003), J. Mol. Biol., 333, 817-829.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.91 expressed in Escherichia coli Trichoderma reesei

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.1.91 hanging drop vapor diffusion method Trichoderma reesei

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.91 D241C/D249C thermostability similar to wild-type enzyme Trichoderma reesei
3.2.1.91 DELTAG245-Y252 reduced thermostability Trichoderma reesei
3.2.1.91 Y247F slight reduction of kcat on 4-nitrophenyl lactoside, but only a small effect on cellulose hydrolysis Trichoderma reesei

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.91 cellobiose competitive inhibitor with 4-nitrophenyl lactoside as substrate Trichoderma reesei

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.91 0.04
-
 4-nitrophenyl lactoside pH 5, 30°C, Y247F mutant Trichoderma reesei
3.2.1.91 0.041
-
 4-nitrophenyl lactoside pH 5, 30°C, wild-type enzyme Trichoderma reesei
3.2.1.91 0.44
-
 4-nitrophenyl lactoside pH 5, 30°C, D241C/D249C mutant Trichoderma reesei
3.2.1.91 1.8
-
 4-nitrophenyl lactoside pH 5, 30°C, DELTAG245-Y252 mutant Trichoderma reesei

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.91 Trichoderma reesei P62694
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.91 4-nitrophenyl beta-lactoside + H2O
-
 Trichoderma reesei 4-nitrophenol + ?
-
 ?
3.2.1.91 cellulose + H2O
-
 Trichoderma reesei ?
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.91 0.061
-
 4-nitrophenyl lactoside pH 5, 30°C, Y247F mutant Trichoderma reesei
3.2.1.91 0.093
-
 4-nitrophenyl lactoside pH 5, 30°C, wild-type enzyme Trichoderma reesei
3.2.1.91 0.19
-
 4-nitrophenyl lactoside pH 5, 30°C, DELTAG245-Y252 mutant Trichoderma reesei
3.2.1.91 0.5
-
 cellotetraose pH 5, 27°C, wild-type enzyme Trichoderma reesei
3.2.1.91 0.58
-
 cellotetraose pH 5, 27°C, Y247F mutant and D241C/D249C mutant Trichoderma reesei
3.2.1.91 0.82
-
 4-nitrophenyl lactoside pH 5, 30°C, D241C/D249C mutant Trichoderma reesei
3.2.1.91 0.83
-
 cellotetraose pH 5, 27°C, DELTAG245-Y252 mutant Trichoderma reesei

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.2.1.91 22
-
 cellobiose pH 5, 30°C, Y247F mutant Trichoderma reesei
3.2.1.91 24
-
 cellobiose pH 5, 30°C, wild-type enzyme Trichoderma reesei
3.2.1.91 300
-
 cellobiose pH 5, 30°C, DELTAG245-Y252 mutant Trichoderma reesei