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Literature summary extracted from

  • Couture, J.F.; Legrand, P.; Cantin, L.; Luu-The, V.; Labrie, F.; Breton, R.
    Human 20alpha-hydroxysteroid dehydrogenase: crystallographic and site-directed mutagenesis studies lead to the identification of an alternative binding site for C21-steroids (2003), J. Mol. Biol., 331, 593-604.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.149 ternary complex with NADP+ and 20alpha-hydroxyprogesterone Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.149 D309L similar Km-value as wild-type Homo sapiens
1.1.1.149 E127D increase in Km-value Homo sapiens
1.1.1.149 H222I dramatic increase in Km-value Homo sapiens
1.1.1.149 H222S large increase in Km-value Homo sapiens
1.1.1.149 R304L dramatic increase in Km-value Homo sapiens
1.1.1.149 T307V similar Km-value as wild-type Homo sapiens
1.1.1.149 Y305F similar Km-value as wild-type Homo sapiens
1.1.1.149 Y55F no enzymic activity Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.149 0.0002
-
 NADPH wild type, pH 7.5, 37°C Homo sapiens
1.1.1.149 0.00117
-
 20alpha-hydroxyprogesterone wild type, pH 7.5, 37°C Homo sapiens
1.1.1.149 0.0021
-
 NADPH mutant H222S, pH 7.5, 37°C Homo sapiens
1.1.1.149 0.0041
-
 progesterone mutant T307V, pH 7.5, 37°C Homo sapiens
1.1.1.149 0.0042
-
 progesterone wild type, pH 7.5, 37°C Homo sapiens
1.1.1.149 0.0046
-
 progesterone mutant D309L, pH 7.5, 37°C Homo sapiens
1.1.1.149 0.0056
-
 progesterone mutant Y305F, pH 7.5, 37°C Homo sapiens
1.1.1.149 0.0094
-
 progesterone mutant E127D, pH 7.5, 37°C Homo sapiens
1.1.1.149 0.019
-
 NADPH mutant H222S, pH 7.5, 37°C Homo sapiens
1.1.1.149 0.0256
-
 progesterone mutant H222S, pH 7.5, 37°C Homo sapiens
1.1.1.149 0.0286
-
 progesterone mutant H222I, pH 7.5, 37°C Homo sapiens
1.1.1.149 0.069
-
 dihydrotestosterone wild type, pH 7.5, 37°C Homo sapiens
1.1.1.149 0.098
-
 20alpha-hydroxyprogesterone mutant H222I, pH 7.5, 37°C Homo sapiens
1.1.1.149 0.151
-
 dihydrotestosterone mutant R304L, pH 7.5, 37°C Homo sapiens
1.1.1.149 0.1774
-
 20alpha-hydroxyprogesterone mutant H222S, pH 7.5, 37°C Homo sapiens
1.1.1.149 0.2833
-
 progesterone mutant R304L, pH 7.5, 37°C Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.149 Homo sapiens
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.149 20alpha-hydroxyprogesterone + NADP+
-
 Homo sapiens progesterone + NADPH
-
 r
1.1.1.149 dihydrotestosterone + NADPH
-
 Homo sapiens ? + NADP+
-
 r
1.1.1.149 progesterone + NADPH
-
 Homo sapiens 20alpha-hydroxyprogesterone + NADP+
-
 r

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.149 NADP+
-
 Homo sapiens
1.1.1.149 NADPH
-
 Homo sapiens