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Literature summary extracted from

  • Kaye, N.M.; Zahler, N.H.; Christian, E.L.; Harris, M.E.
    Conservation of helical structure contributes to functional metal ion interactions in the catalytic domain of ribonuclease P RNA (2002), J. Mol. Biol., 324, 429-442.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
3.1.26.7 additional information construction of ribozyme mutants with moved bulged nucleotides important for metal ion coordination, proximal or distal to metal coordination sites, the first has moderate but positive effect on catalysis and cleavage pattern, the latter has large, negative effects on catalysis and cleavage pattern, overview Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.26.7 additional information
-
 additional information kinetics, wild-type and mutant ribozymes Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.26.7 Mg2+ required, potential metal binding sites identification by Tb3+ cleavage Escherichia coli
3.1.26.7 additional information conservation of helical structure contributes to functional metal ion interactions in the catalytic domain of the ribozyme Escherichia coli
3.1.26.7 NaCl
-
 Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
3.1.26.7 Escherichia coli
-
 catalytic RNA
-

Reaction

EC Number Reaction Comment Organism Reaction ID
3.1.26.7 endonucleolytic cleavage of RNA, removing 3'-extranucleotides from tRNA precursor cleavage sites and pattern Escherichia coli
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.26.7 pre-tRNA + H2O structure-function relationship, moving the bulged nucleotides proximal to sites of metal ion coordination in helix P4 is important for catalysis, overview Escherichia coli tRNA + pre-part nucleotides
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.26.7 ribonuclease P RNA
-
 Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.26.7 37
-
 assay at, standard trans-conditions Escherichia coli
3.1.26.7 50
-
 assay at, standard cis-conditions Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.26.7 5.5
-
 assay at, standard cis-conditions Escherichia coli
3.1.26.7 6
-
 assay at, standard trans-conditions Escherichia coli